College of Agriculture, Jilin Agricultural University, Changchun, China.
Crop Science Post-doctoral Station, Jilin Agricultural University, Changchun, China.
Physiol Plant. 2024 Nov-Dec;176(6):e14615. doi: 10.1111/ppl.14615.
Northern corn leaf blight, caused by Exserohilum turcicum (E. turcicum), is one of the most destructive diseases in maize, leading to serious yield losses. However, the underlying molecular mechanisms of E. turcicum infection response in maize remain unclear. In this study, we performed comparative transcriptome analysis in resistant maize inbred line J9D207 (R) and susceptible maize inbred line PH4CV (S) after infecting with E. turcicum at 0 h, 24 h and 72 h, respectively. Compared with 0 h, 9656 (24 h) and 8748 (72 h) differentially expressed genes (DEGs) were identified in J9D207, and 7915 (24 h) and 7865 (72 h) DEGs were identified in PH4CV. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that alpha-linolenic acid metabolism, benzoxazinoid biosynthesis, flavonoid biosynthesis and phenylpropanoid biosynthesis might be involved in maize defense reactions. Some DEGs coded for transcription factors, such as MYB-related, ERF, NAC, bZIP, bHLH and WRKY families, which indicated that they may participate in resistance against E. turcicum. In addition, DEGs involved in SA, JA, ABA and ET signaling pathways were revealed. Moreover, 75 SOD activity-related genes and 421 POD activity-related genes were identified through weighted gene co-expression network analysis (WGCNA), respectively. These results provide a novel insight into the resistance mechanism of maize in response to E. turcicum inoculation.
北方玉米叶枯病由突脐蠕孢菌（E. turcicum）引起，是玉米最具破坏性的病害之一，导致严重的产量损失。然而，玉米突脐蠕孢菌感染反应的潜在分子机制尚不清楚。在这项研究中，我们分别在 E. turcicum 接种后 0 h、24 h 和 72 h 时，对抗性玉米自交系 J9D207（R）和感病玉米自交系 PH4CV（S）进行了比较转录组分析。与 0 h 相比，J9D207 中分别有 9656 个（24 h）和 8748 个（72 h）差异表达基因（DEGs），PH4CV 中分别有 7915 个（24 h）和 7865 个（72 h）DEGs。京都基因与基因组百科全书（KEGG）富集分析表明，α-亚麻酸代谢、苯并恶嗪生物合成、类黄酮生物合成和苯丙素生物合成可能参与了玉米的防御反应。一些 DEGs 编码转录因子，如 MYB 相关、ERF、NAC、bZIP、bHLH 和 WRKY 家族，表明它们可能参与了对 E. turcicum 的抗性。此外，还揭示了参与 SA、JA、ABA 和 ET 信号通路的 DEGs。此外，通过加权基因共表达网络分析（WGCNA）分别鉴定了 75 个 SOD 活性相关基因和 421 个 POD 活性相关基因。这些结果为玉米对 E. turcicum 接种的抗性机制提供了新的见解。
