基于硫量子点和 MnO 纳米片的“开启”型检测和成像谷胱甘肽的高灵敏快速荧光传感器。

A Sensitive and Quick Fluorescent Sensor for the "Turn-On" Detection and Imaging of Glutathione Based on Sulfur Quantum Dots and MnO Nanosheets.

机构信息

College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

Fujian Key Laboratory of Integrative Medicine on Geriatrics, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China.

出版信息

Luminescence. 2024 Nov;39(11):e4929. doi: 10.1002/bio.4929.

DOI:10.1002/bio.4929

PMID:39508153

Abstract

Glutathione (GSH) is one of the most abundant bioethanol antioxidants in living cells. Here, a fluorescent probe based on MnO nanosheets and sulfur quantum dots (SQDs) was fabricated. Because of the synergistic effect of IFE and FRET, the fluorescence from SQDs was quenched by MnO nanosheets. In the presence of GSH, the fluorescence of SQDs could be recovered because of the reduction of MnO nanosheets by GSH. The method can detect GSH in the concentration range of 5 ~ 1000 μM with the detection limit as low as 1.26 μM. This quick, easy, and cost-effective sensor could be used for the quantification of GSH in serum samples and the imaging of GSH in Escherichia coli O157:H7.

摘要

谷胱甘肽（GSH）是活细胞中含量最丰富的生物乙醇抗氧化剂之一。在这里，我们制备了一种基于 MnO 纳米片和硫量子点（SQDs）的荧光探针。由于内滤效应（IFE）和荧光能量转移（FRET）的协同作用，MnO 纳米片使 SQDs 的荧光猝灭。在 GSH 的存在下，由于 GSH 将 MnO 纳米片还原，SQDs 的荧光可以恢复。该方法可以在 5 ～ 1000 μM 的浓度范围内检测 GSH，检测限低至 1.26 μM。这种快速、简单且具有成本效益的传感器可用于血清样品中 GSH 的定量检测和大肠杆菌 O157:H7 中 GSH 的成像。