College of Chemistry, Fuzhou University, Fuzhou, 350116, Fujian, China.
College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350116, Fujian, China.
Anal Bioanal Chem. 2020 Nov;412(29):8167-8176. doi: 10.1007/s00216-020-02951-8. Epub 2020 Sep 15.
Fluorescence biosensors that enable highly sensitive detection of glutathione (GSH) are in great demand for various biological investigations and early disease diagnoses. Here, we report a turn-on fluorescence nanoplatform based on fluorescent semiconducting polymer nanoparticle@MnO nanosheets (P-dot@MnO) nanocomposites for rapid homogeneous determination of GSH. The near-infrared (NIR) fluorescent P-dots were prepared by doping NIR dyes into polymer matrix and then encompassed by MnO, which resulted in a remarkable fluorescence quenching. Owing to the selective decomposition of MnO by GSH, the fluorescence recovery was achieved in the presence of GSH. On the basis of the target-induced turn-on fluorescence response, the developed nanoplatform can readily detect GSH with a high sensitivity up to 0.26 μM, as well as a superior specificity. Furthermore, it was successfully applied in monitoring the intracellular GSH in living cells, revealing its great potential in biomedical applications. Graphical abstract.
荧光生物传感器能够高灵敏度地检测谷胱甘肽 (GSH)，因此在各种生物研究和早期疾病诊断中都有很大的需求。在这里，我们报告了一种基于荧光半导体聚合物纳米粒子@MnO 纳米片 (P-dot@MnO) 纳米复合材料的荧光纳米平台，用于快速均相测定 GSH。通过将近红外 (NIR) 荧光染料掺杂到聚合物基质中，然后将其包裹在 MnO 中，制备了近红外 (NIR) 荧光 P 点，从而导致荧光显著猝灭。由于 GSH 选择性地分解 MnO，因此在存在 GSH 的情况下实现了荧光恢复。基于目标诱导的荧光开启响应，所开发的纳米平台可以很容易地检测 GSH，灵敏度高达 0.26 μM，并且具有优异的特异性。此外，它还成功地应用于监测活细胞内的 GSH，显示出其在生物医学应用中的巨大潜力。
