College of Plant Protection, China Agricultural University, Beijing 100193, China.
Science and Technology Achievement Transformation Management Office, Yunnan Academy of Agricultural Sciences, Kunming 650224, China.
J Agric Food Chem. 2024 Nov 20;72(46):25490-25499. doi: 10.1021/acs.jafc.4c06416. Epub 2024 Nov 7.
The oriental fruit fly, (Hendel), is a highly invasive and destructive pest. Chlorfenapyr is a widely used insecticide that disrupts mitochondrial activity. The Parkin protein plays conserved roles in maintaining mitochondrial homeostasis, but the role of Parkin in response to chlorfenapyr remains largely unknown. Here, we report that BdParkin is required for chlorfenapyr detoxification, and dsRNA targeting improves the insecticidal efficacy of chlorfenapyr. Among the genes whose expression levels are affected by RNAi, knock-down of the glutathione S-transferase gene increases the insecticidal efficacy of chlorfenapyr. Molecular docking reveals potential interactions between BdGSTd2 and tralopyril, an insecticidal metabolite of chlorfenapyr. These results suggest that BdParkin could impact the response of to chlorfenapyr through metabolic processes regulated by BdGSTd2. Our findings could offer new insights into how insects detoxify chlorfenapyr and provide molecular targets for developing a sustainable management strategy for .
东方果实蝇（Hendel）是一种极具侵袭性和破坏性的害虫。氯氟氰菊酯是一种广泛使用的杀虫剂，它会破坏线粒体的活动。Parkin 蛋白在维持线粒体稳态方面发挥着保守作用，但 Parkin 在应对氯氟氰菊酯方面的作用在很大程度上仍然未知。在这里，我们报告说 BdParkin 是氯氟氰菊酯解毒所必需的，靶向的 dsRNA 可提高氯氟氰菊酯的杀虫效果。在表达水平受 RNAi 影响的基因中，敲低谷胱甘肽 S-转移酶基因 可提高氯氟氰菊酯的杀虫效果。分子对接显示 BdGSTd2 与氯氟氰菊酯的杀虫代谢物 tralopyril 之间存在潜在相互作用。这些结果表明，BdParkin 可能通过 BdGSTd2 调节的代谢过程影响 对氯氟氰菊酯的反应。我们的研究结果为昆虫如何解毒氯氟氰菊酯提供了新的见解，并为开发 的可持续管理策略提供了分子靶标。
