Liu Zhongkai, Huang Jin, Liu Mengqi, Cui Liying, Li Xiaoyu, Meng Qi, Wang Xiaoshuai, Liu Shengkai, Peng Jinsong, Liu Zhiguo
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, People's Republic of China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China; Heilongjiang Provincial Key Laboratory of Ecological Utilization Of Forestry-based Active Substances, People's Republic of China.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, People's Republic of China.
Colloids Surf B Biointerfaces. 2025 Jan;245:114357. doi: 10.1016/j.colsurfb.2024.114357. Epub 2024 Nov 6.
In this study, we developed the ginger vesicles as nanocarrier for the targeted delivery of 10-hydroxy-camptothecin (HCPT), aiming to improve its therapeutic efficacy while minimizing the systemic toxicity. Ginger vesicles exhibit a wide spectrum of biological activities and excellent biocompatibility, rendering them as the promising nanocarriers candidates for anticancer drug delivery. The ginger vesicles with an average diameter of 86.83 nm were successfully prepared by utilizing a gradient centrifugation method. The loading conditions for HCPT into the ginger vesicles were optimized through the addition of an appropriate amount of Ca. The loading efficiency, size distribution, stability, and cytotoxicity profile of the ginger vesicles were comprehensively characterized using UV spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), and cytotoxicity experiments. Furthermore, in vitro cytotoxicity studies confirmed that ginger vesicles loaded with HCPT exhibited high inhibitory activity against tumor cells as evidenced by fluorescence imaging and flow cytometry analysis. Most importantly, in vivo antitumor assay demonstrated that the ginger vesicles loaded with HCPT displayed remarkable inhibitory effects on tumor growth. In summary, our results demonstrated the potential application of the ginger vesicles as ideal nanocarriers for delivering HCPT.
在本研究中，我们开发了姜泡囊作为纳米载体用于靶向递送10-羟基喜树碱（HCPT），旨在提高其治疗效果同时将全身毒性降至最低。姜泡囊具有广泛的生物活性和优异的生物相容性，使其成为抗癌药物递送的有前景的纳米载体候选物。通过梯度离心法成功制备了平均直径为86.83 nm的姜泡囊。通过添加适量的钙优化了HCPT载入姜泡囊的条件。使用紫外光谱、透射电子显微镜（TEM）、动态光散射（DLS）和细胞毒性实验对姜泡囊的载入效率、粒径分布、稳定性和细胞毒性特征进行了全面表征。此外，体外细胞毒性研究证实，载有HCPT的姜泡囊对肿瘤细胞表现出高抑制活性，荧光成像和流式细胞术分析证明了这一点。最重要的是，体内抗肿瘤试验表明，载有HCPT的姜泡囊对肿瘤生长显示出显著的抑制作用。总之，我们的结果证明了姜泡囊作为递送HCPT的理想纳米载体的潜在应用。
