金鸡菊芽的梯度乙醇提取物：化学组成和体外发酵特性。

Gradient ethanol extracts of Coreopsis tinctoria buds: Chemical and in vitro fermentation characteristics.

机构信息

College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang 524088, China; Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832003, China; School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.

School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.

出版信息

Food Chem. 2025 Feb 1;464(Pt 3):141894. doi: 10.1016/j.foodchem.2024.141894. Epub 2024 Nov 1.

DOI:10.1016/j.foodchem.2024.141894

PMID:39509897

Abstract

Coreopsis tinctoria buds grow at high altitudes and have unique medicinal and health effects. This study focused on the chemical components of gradient ethanol extracts of Coreopsis tinctoria buds and their impact on the gut microbiota and its metabolites. UPLC-Q-Exactive-MS results disclosed the presence of 24 distinct chemicals in the extracts, marein, and quercetin-3-β-D-glucoside as the predominant antioxidants. Furthermore, the anhydrous ethanol extract (EtOH-EX) and 80 % (w/w) ethanol extract (80 % EtOH-EX) showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging capacity, and the water extract (Water-Ex) showed excellent bile acid binding capacity. Besides, simulated fermentation of the extracts increased short-chain fatty acids and the abundance of gut-friendly bacteria like Bifidobacteria, especially in the Water-EX group. Correlation analysis revealed that the key microbiota on metabolites production. In conclusion, the extracts derived from Coreopsis tinctoria buds exhibit a spectrum of bioactivities, viewing them as flexible options for applications across food and pharmaceutical products.

摘要

金鸡菊芽生长在高海拔地区，具有独特的药用和保健功效。本研究专注于金鸡菊芽梯度乙醇提取物的化学成分及其对肠道微生物群及其代谢物的影响。UPLC-Q-Exactive-MS 结果显示提取物中存在 24 种不同的化学物质，马雷因和槲皮素-3-β-D-葡萄糖苷为主要的抗氧化剂。此外，无水乙醇提取物（EtOH-EX）和 80%（w/w）乙醇提取物（80%EtOH-EX）表现出最高的 1,1-二苯基-2-苦基肼（DPPH）和 2,2'-联氮双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除能力，而水提取物（Water-EX）表现出出色的胆汁酸结合能力。此外，提取物的模拟发酵增加了短链脂肪酸和双歧杆菌等肠道友好细菌的丰度，尤其是在 Water-EX 组。相关分析显示，关键微生物群对代谢产物的产生有影响。总之，金鸡菊芽提取物具有多种生物活性，可作为食品和医药产品应用的灵活选择。