Curtis M J, Macleod B A, Tabrizchi R, Walker M J
J Pharmacol Methods. 1986 Feb;15(1):87-94. doi: 10.1016/0160-5402(86)90008-2.
A perfusion apparatus for mechanical and electrophysiological studies in small animal (e.g., rat, guinea pig) hearts is described. The apparatus consists of a number of individual perfusion chambers connected to the aortic perfusion cannula of a Langendorff perfused heart. The aortic root perfusion pressure is controlled by the oxygenating gas (usually 5% CO2 in O2) at a range of 0-200 mmHg. Different solutions can be placed in different individual chambers. Pressure in the left ventricle is monitored by means of a special compliant, but nonelastic, balloon while special atraumatic electrodes allow high-voltage, noise-free ECG recordings to be made from all parts of the epicardium. The apparatus keeps hearts beating for hours while the multichamber allows multiple drug applications to be made rapidly. Thus, the apparatus can be used for all types of pharmacodynamic analysis.
本文描述了一种用于小动物（如大鼠、豚鼠）心脏机械和电生理研究的灌注装置。该装置由多个独立的灌注室组成，这些灌注室连接到Langendorff灌注心脏的主动脉灌注插管。主动脉根部灌注压力由含氧气体（通常为氧气中含5%二氧化碳）控制，范围为0 - 200 mmHg。不同的溶液可放置在不同的独立腔室中。左心室压力通过一个特殊的顺应性但无弹性的球囊进行监测，而特殊的无创电极允许从心外膜的所有部位进行高电压、无噪声的心电图记录。该装置可使心脏跳动数小时，而多腔室允许快速进行多种药物应用。因此，该装置可用于所有类型的药效学分析。
