Krzemiński T, Kurcok A, Kapustecki J, Kowaliński J, Slowiński Z, Brus R
Department of Pharmacology, Silesian Academy of Medicine, Zabrze, Poland.
J Pharmacol Methods. 1991 Apr;25(2):95-110. doi: 10.1016/0160-5402(91)90001-l.
A modified perfusion apparatus for electrophysiological, hemodynamic and metabolic studies on hearts of small animal (e.g., rat, guinea pig) has been described. The apparatus consisted of two oxygenators connected to the aortic cannula of the Langendorff perfused heart, each one of which could be used on demand. The constant aortic perfusion pressure was controlled by oxygenating gas (5% CO2 in O2, usually). The perfusing medium could be changed easily and quickly without interruption. Left ventricular pressure was recorded by means of a balloon-catheter, while special suction electrodes obtained the high-amplitude, noise-free electrogram recordings. The coronary effluent partial pressure of oxygen (Po2) was continuously monitored, which enabled the calculation of myocardial oxygen consumption (MVO2). The effluent partial pressure of carbon dioxide (Pco2) and pH value were also measured simultaneously. A computerized system of data acquisition, calculation, storage, and end report has also been described. In this study, the influence of indomethacin on hypoxia-induced hemodynamic and metabolic changes in the isolated guinea pig heart was observed. The advantages and disadvantages of the described and standard Langendorff apparatus have been discussed also.
一种用于小动物（如大鼠、豚鼠）心脏电生理、血流动力学和代谢研究的改良灌注装置已被描述。该装置由两个与Langendorff灌注心脏的主动脉插管相连的氧合器组成，每个氧合器可按需使用。恒定的主动脉灌注压力通常由含氧气体（通常为氧气中含5%二氧化碳）控制。灌注介质可在不中断的情况下轻松快速更换。左心室压力通过球囊导管记录，而特殊的吸引电极可获得高振幅、无噪声的心电图记录。连续监测冠状动脉流出液的氧分压（Po2），从而能够计算心肌耗氧量（MVO2）。同时还测量流出液的二氧化碳分压（Pco2）和pH值。还描述了一个数据采集、计算、存储和最终报告的计算机系统。在本研究中，观察了吲哚美辛对离体豚鼠心脏缺氧诱导的血流动力学和代谢变化的影响。还讨论了所述改良装置与标准Langendorff装置的优缺点。
