Rezai Shaghayegh, Ghorbani Elnaz, Khazaei Majid, Nazari Seyedeh Elnaz, Rahmani Farzad, Naimi Hamideh, Afshari Asma, Avan Amir, Ryzhikov Mikhail, Soleimanpour Saman, Mehr Seyed Mahdi Hasanian
Department of Medical Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Medical Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Adv Biomed Res. 2024 Sep 23;13:85. doi: 10.4103/abr.abr_157_24. eCollection 2024.
This investigation investigates the anti-inflammatory and fibrinolytic effects of a cocktail of probiotics derived from traditional dairy products in a murine model of ulcerative colitis (UC).
A mix of newly isolated probiotics containing , and was characterized and orally administered to inbred eight-week-old C57BL/6 male mice (n = 6). Clinical symptoms, pathohistological changes, and inflammatory and fibrosis markers were analyzed in the existence and absence of probiotics in colitis mice.
Dairy probiotics potently attenuated colitis symptoms by decreasing dextran sulfate sodium (DSS)-induced body weight loss, colon shortening, rectal bleeding, and rectal prolapse. Consistently, a cocktail of probiotics could significantly improve histopathological grading by suppressing crypt loss, mucosal damage, and inflammation scores in colitis tissues. Moreover, the mix of probiotics suppressed pro-inflammatory genes including interleukin (IL)-1β, IL-6, tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ), and increased anti-oxidant markers and activity such as superoxide dismutase and catalase in colon tissue. Furthermore, compared to the no-treated group, the administration of probiotics reduced fibrosis by decreasing collagen deposition in tissue sections and down-regulating levels of pro-fibrotic genes including alpha-actin-2 (Acta2), collagen (Col) 1a1, and Col 1a2 in colitis tissue homogenates.
The results show the newly isolated cocktail of probiotics elicits a potent protective effect on UC symptoms in mice model. Further study on these probiotics is required to fully explore their effectiveness, strength, and safety considerations.
本研究在溃疡性结肠炎（UC）小鼠模型中研究了源自传统乳制品的益生菌混合物的抗炎和纤维蛋白溶解作用。
对一种新分离的含有[具体菌株]的益生菌混合物进行了表征，并将其口服给予8周龄的近交系C57BL/6雄性小鼠（n = 6）。分析了结肠炎小鼠在有或没有益生菌存在的情况下的临床症状、病理组织学变化以及炎症和纤维化标志物。
乳制品益生菌通过减少硫酸葡聚糖钠（DSS）诱导的体重减轻、结肠缩短、直肠出血和直肠脱垂，有效减轻了结肠炎症状。一致地，益生菌混合物可以通过抑制结肠炎组织中的隐窝丢失、粘膜损伤和炎症评分，显著改善组织病理学分级。此外，益生菌混合物抑制了包括白细胞介素（IL）-1β、IL-6、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）在内的促炎基因，并增加了结肠组织中的抗氧化标志物和超氧化物歧化酶和过氧化氢酶等活性。此外，与未治疗组相比，给予益生菌通过减少组织切片中的胶原蛋白沉积和下调结肠炎组织匀浆中包括α-肌动蛋白-2（Acta2）、胶原蛋白（Col）1a1和Col 1a2在内的促纤维化基因水平，减少了纤维化。
结果表明，新分离的益生菌混合物对小鼠模型中的UC症状具有强大的保护作用。需要对这些益生菌进行进一步研究，以充分探索它们的有效性、强度和安全性考虑因素。
