Centre for Host-Microbiome Interactions, Faculty of Dentistry, Oral and Craniofacial Sciences, King's College London, London SE1 9RT, UK.
Int J Mol Sci. 2024 Oct 28;25(21):11552. doi: 10.3390/ijms252111552.
Three-dimensional organotypic models benefit from the ability to mimic physiological cell-cell or cell-matrix interactions and therefore offer superior models for studying pathological or physiological conditions compared to 2D cultures. Organotypic models consisting of keratinocytes supported by fibroblasts embedded in collagen matrices have been utilised for the study of oral conditions. However, the provision of a suitable model for investigating the pathogenesis of periodontitis has been more challenging. Part of the complexity relates to the different regional epithelial specificities and connective tissue phenotypes. Recently, it was confirmed, using 3D organotypic models, that distinct fibroblast populations were implicated in the provision of specific inductive and directive influences on the overlying epithelia. This paper presents the organotypic model of the dentogingival junction (DGJ) constructed to demonstrate the differential fibroblast influences on the maintenance of regionally specific epithelial phenotypes. Therefore, the review aims are (1) to provide the biological basis underlying 3D organotypic cultures and (2) to comprehensively detail the experimental protocol for the construction of the organotypic cultures and the unique setup for the DGJ model. The latter is the first organotypic culture model used for the reconstruction of the DGJ and is recommended as a useful tool for future periodontal research.
三维器官型模型受益于模拟生理细胞-细胞或细胞-基质相互作用的能力,因此与 2D 培养相比,为研究病理或生理条件提供了更好的模型。由成纤维细胞支持的角质形成细胞组成的器官型模型已被用于口腔状况的研究。然而,提供一个合适的模型来研究牙周炎的发病机制更具挑战性。部分复杂性与不同的区域上皮特异性和结缔组织表型有关。最近,使用三维器官型模型证实,不同的成纤维细胞群体参与了对上皮的特定诱导和定向影响。本文介绍了构建的牙龈结合部(DGJ)器官型模型,以证明成纤维细胞对维持区域特异性上皮表型的不同影响。因此,本综述的目的是(1)提供 3D 器官型培养的生物学基础,(2)全面详细介绍器官型培养的构建实验方案,以及 DGJ 模型的独特设置。后者是第一个用于重建 DGJ 的器官型培养模型,被推荐为未来牙周研究的有用工具。