抗生物素蛋白基因的分子克隆与核苷酸序列
Molecular cloning and nucleotide sequence of the streptavidin gene.
作者信息
Argaraña C E, Kuntz I D, Birken S, Axel R, Cantor C R
出版信息
Nucleic Acids Res. 1986 Feb 25;14(4):1871-82. doi: 10.1093/nar/14.4.1871.
Abstract
Using synthetic oligonucleotides as probes we have cloned the streptavidin gene from a genomic library of Streptomyces avidinii. Nucleotide sequence analysis indicated that a 2 Kb DNA-fragment contained the entire coding region, a signal peptide region and the 3' and 5' flanking regions of the gene. The deduced amino acid sequence shows several interrupted blocks of homology with the amino acid sequence of chicken egg-white avidin. Analysis of the secondary structure suggests a high content of beta-structure in both proteins and considerable overall structural similarity between them.
摘要
我们使用合成寡核苷酸作为探针，从嗜热栖热放线菌的基因组文库中克隆了抗生物素蛋白基因。核苷酸序列分析表明，一个2kb的DNA片段包含了整个编码区、一个信号肽区以及该基因的3'和5'侧翼区。推导的氨基酸序列显示与鸡卵抗生物素蛋白的氨基酸序列有几个间断的同源性区域。二级结构分析表明，这两种蛋白质中β结构的含量都很高，并且它们之间在整体结构上有相当大的相似性。
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