Nagarajan V, Ramaley R, Albertson H, Chen M
Central Research and Development Division, E. I. duPont de Nemours Company, Wilmington, Delaware 19880-0328.
Appl Environ Microbiol. 1993 Nov;59(11):3894-8. doi: 10.1128/aem.59.11.3894-3898.1993.
Streptavidin is an extracellular tetrameric protein produced by Streptomyces avidinii. A series of hybrid gene fusions consisting of Bacillus signal peptide coding regions fused to the mature streptavidin sequence were constructed. B. subtilis strains harboring these plasmids accumulate a tetrameric streptavidin in the growth medium. The properties of the streptavidin produced by B. subtilis are similar to those of the streptavidin produced by S. avidinii. B. subtilis strains carrying the various fusions can be grown to a high cell density in a biotin-free medium. Thus, B. subtilis represents an alternate host system for the production of streptavidin.
链霉亲和素是由抗生链霉菌产生的一种细胞外四聚体蛋白。构建了一系列由芽孢杆菌信号肽编码区与成熟链霉亲和素序列融合而成的杂交基因融合体。携带这些质粒的枯草芽孢杆菌菌株在生长培养基中积累四聚体链霉亲和素。枯草芽孢杆菌产生的链霉亲和素的特性与抗生链霉菌产生的链霉亲和素相似。携带各种融合体的枯草芽孢杆菌菌株可以在无生物素的培养基中生长至高细胞密度。因此，枯草芽孢杆菌代表了生产链霉亲和素的另一种宿主系统。
