Key Laboratory of Intelligent Organic Tea Garden Construction in Universities of Yunnan Province, Yunnan Organic Tea Industry Intelligent Engineering Research Center, Yunnan Agricultural University, Kunming 650201, China; School of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan 610106, China.
Key Laboratory of Intelligent Organic Tea Garden Construction in Universities of Yunnan Province, Yunnan Organic Tea Industry Intelligent Engineering Research Center, Yunnan Agricultural University, Kunming 650201, China.
Bioresour Technol. 2025 Jan;416:131751. doi: 10.1016/j.biortech.2024.131751. Epub 2024 Nov 7.
In this study, we successfully generated the mutant strain Bacillus tropicus AT31-1 from AT31 through atmospheric room-temperature plasma mutagenesis. This mutant strain AT31-1 demonstrated an impressive 48.6 % removal efficiency in 400 mg/L lead medium. Comparative genomic analysis showed that the mutant strain AT31-1 had three mutation sites, which affect the efflux RND transporter permease subunit, the response regulator transcription factor, and a gene with unknown function. The transcriptional analysis showed a notable upregulation in the expression of 283 genes in AT31-1 as lead concentrations increased from 0 to 200 mg/L and then to 400 mg/L, which include zinc-transporting ATPase, ferrous iron transport protein B, NADH dehydrogenase, and others. The Gene ontology function of the peptide metabolic process, along with the KEGG pathway of carbon metabolism were identified as closely linked to the extreme lead tolerance of AT31-1. This study presents novel insights into the lead tolerance mechanisms of bacteria.
在本研究中，我们通过大气室温等离子体诱变成功地从 AT31 中生成了突变株 Bacillus tropicus AT31-1。该突变株 AT31-1 在 400mg/L 铅介质中表现出令人印象深刻的 48.6%的去除效率。比较基因组分析表明，突变株 AT31-1 有三个突变位点，影响外排 RND 转运体通透酶亚基、应答调节转录因子和一个功能未知的基因。转录分析表明，随着铅浓度从 0 增加到 200mg/L，再增加到 400mg/L，AT31-1 中 283 个基因的表达显著上调，其中包括锌转运 ATP 酶、亚铁运输蛋白 B、NADH 脱氢酶等。肽代谢过程的基因本体功能以及碳代谢的 KEGG 途径被确定与 AT31-1 的极端铅耐受性密切相关。本研究为细菌的铅耐受机制提供了新的见解。
