Fe-based nanozyme with photothermal activity prepared from polymerization-induced self-assembly assays boosts the recovery of bacteria-infected wounds.

Nowadays, the overuse of antibiotics has escalated bacterial infections into an increasingly severe global health threat. Developing non-antibiotic treatments has emerged as a promising strategy for treating bacterial infections. Notably, nanozyme-based composite materials have garnered growing interest. Therefore, the efficient preparation of nanozyme is important. Herein, we have presented an efficient method to prepare Fe-based nanozyme through polymerization-induced self-assembly assay to kill bacteria efficiently, which could significantly enhance the healing of infected wounds. Through polymerization-induced self-assembly assay, a large number of uniformly sized micelles, bearing imidazole groups, could be efficiently prepared. These nanoparticles subsequently chelate with Fe ions, followed by pyrolysis and etching processes, resulting in the production of uniformly small-sized nanozymes with high adsorption activity in the near-infrared region. The composite materials could effectively eradicate bacteria via a synergistic strategy of photothermal and catalytic therapies under infected microenvironments. In vivo animal models with full-thickness wounds showed that combination therapy not only eradicates 98 % of the bacteria but also significantly accelerates wound healing. This work underscores the utility of polymerization-induced self-assembly in the preparation of nanozymes and reveals promising applications of nanozymes in wound healing. STATEMENT OF SIGNIFICANCE: This research introduces a functional nanozyme with photothermal activity, synthesized through polymerization-induced self-assembly, offering a promising non-antibiotic strategy to combat bacterial infections. This strategy enhances wound healing by combining photothermal and catalytic therapies, effectively eradicating drug-resistant bacteria while minimizing damage to healthy tissue. Our findings hold significant implications for the development of advanced antibacterial treatments and offer a robust assay to prepare nanozyme with small sizes. The prepared functional nanoparticles have a potential in wound healing, addressing a critical need in the face of rising antibiotic resistance.