Stensvold Christen Rune
Laboratory of Parasitology, Statens Serum Institut, Copenhagen, Denmark; Department of Protozoology, Mahidol University, Bangkok, Thailand.
Trends Parasitol. 2024 Dec;40(12):1173-1182. doi: 10.1016/j.pt.2024.10.015. Epub 2024 Nov 8.
Next-generation sequencing (NGS) methods include whole-genome sequencing, metagenomic analysis, and amplicon-based NGS, all of which are gaining territory in parasitology. A modality of particular interest within the field of gut protozoology is exhaustive metabarcoding of ribosomal genes in a complex matrix such as faeces, by which method, amplicon-based NGS enables the detection and differentiation of both eukaryotic and prokaryotic organisms, circumventing Sanger sequencing-based limitations and representing a one-fits-most approach. Apart from being a tool to break the code of intracellular genetic variation and tell mixed species infections apart, metabarcoding can produce data that can serve to augment our understanding of the interplay between the organisms within the gut.
下一代测序（NGS）方法包括全基因组测序、宏基因组分析和基于扩增子的NGS，所有这些方法在寄生虫学领域都越来越受到关注。肠道原生动物学领域中一种特别有趣的方法是对粪便等复杂基质中的核糖体基因进行详尽的元条形码分析，通过这种基于扩增子的NGS方法，可以检测和区分真核生物和原核生物，克服了基于桑格测序的局限性，是一种适用性很强的方法。除了作为一种破解细胞内基因变异密码和区分混合物种感染的工具外，元条形码分析还可以产生有助于增强我们对肠道内生物体之间相互作用理解的数据。
