Liu X, Yang Y, Liu W, Zhang Z, Zhou X, Xie W, Shen L, Zhang M, Li X, Zang J, Li S
School of Pharmacy, Bengbu Medical University, Bengbu 233030, China.
Anhui Provincial Engineering Technology Research Center of Biochemical Pharmaceuticals, Bengbu 233030, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2024 Oct 20;44(10):1918-1925. doi: 10.12122/j.issn.1673-4254.2024.10.10.
To investigate the effect of on biological behaviors of non-small cell lung cancer (NSCLC) cells.
NSCLC cell lines PC-9 and A549 treated with different concentrations of preparations were examined for changes in proliferation, apoptosis, invasion and migration using CCK-8 assay, colony formation assay, flow cytometry, wound healing assay and Transwell assay. Western blotting was performed to detect the changes in protein expressions of Bax, Bcl-2, E-cadherin, vimentin, MMP2, and MMP9 in the treated cells. PC-9 cells were injected subcutaneously into BALB/C nude mice to establish a nude mouse subcutaneous tumor model. According to the growth of subcutaneous tumors, mice were randomly divided into control group: gavaged daily with saline; -treated group: gavaged daily with 65 mg/mL, and granules were dissolved in saline; cisplatin-treated group: injected intraperitoneally with cisplatin 4 mg/kg every 5 days, 6 mice per group. The subcutaneous tumor volume and mass changes of mice were measured, and the toxic effects of on heart, liver, spleen, lung and kidney as well as the therapeutic effects of were observed in the mice bearing tumor.
granules concentration-dependently inhibited the proliferation and survival of PC-9 and A549 cells, significantly promoted cell apoptosis, suppressed invasion and migration abilities of the cells, up-regulated the expression levels of E-cadherin and Bax, and down-regulated the expressions of Bcl-2, vimentin, MMP2, and MMP9. In the tumor-bearing mice, treatment with significantly inhibited tumor growth without producing obvious toxicity in the vital organs.
can inhibit proliferation, invasion, and migration and induces apoptosis of NSCLC cells .
研究[具体药物或物质]对非小细胞肺癌（NSCLC）细胞生物学行为的影响。
用不同浓度的[具体药物或物质]制剂处理NSCLC细胞系PC-9和A549，采用CCK-8法、集落形成试验、流式细胞术、伤口愈合试验和Transwell试验检测细胞增殖、凋亡、侵袭和迁移的变化。进行蛋白质免疫印迹法检测处理后细胞中Bax、Bcl-2、E-钙黏蛋白、波形蛋白、基质金属蛋白酶2（MMP2）和基质金属蛋白酶9（MMP9）蛋白表达的变化。将PC-9细胞皮下注射到BALB/C裸鼠体内建立裸鼠皮下肿瘤模型。根据皮下肿瘤的生长情况，将小鼠随机分为对照组：每日灌胃生理盐水；[具体药物或物质]治疗组：每日灌胃65 mg/mL的[具体药物或物质]，[具体药物或物质]颗粒溶于生理盐水；顺铂治疗组：每5天腹腔注射顺铂4 mg/kg，每组6只小鼠。测量小鼠皮下肿瘤体积和质量变化，观察[具体药物或物质]对荷瘤小鼠心、肝、脾、肺和肾的毒性作用以及[具体药物或物质]的治疗效果。
[具体药物或物质]颗粒浓度依赖性地抑制PC-9和A549细胞的增殖和存活，显著促进细胞凋亡，抑制细胞的侵袭和迁移能力，上调E-钙黏蛋白和Bax的表达水平，下调Bcl-2、波形蛋白、MMP2和MMP9的表达。在荷瘤小鼠中，[具体药物或物质]治疗显著抑制肿瘤生长，且对重要器官无明显毒性。
[具体药物或物质]可抑制NSCLC细胞的增殖、侵袭和迁移并诱导其凋亡。
