[ inhibits proliferation, invasion, and migration and promotes apoptosis of non-small cell lung cancer cells].

OBJECTIVE

To investigate the effect of on biological behaviors of non-small cell lung cancer (NSCLC) cells.

METHODS

NSCLC cell lines PC-9 and A549 treated with different concentrations of preparations were examined for changes in proliferation, apoptosis, invasion and migration using CCK-8 assay, colony formation assay, flow cytometry, wound healing assay and Transwell assay. Western blotting was performed to detect the changes in protein expressions of Bax, Bcl-2, E-cadherin, vimentin, MMP2, and MMP9 in the treated cells. PC-9 cells were injected subcutaneously into BALB/C nude mice to establish a nude mouse subcutaneous tumor model. According to the growth of subcutaneous tumors, mice were randomly divided into control group: gavaged daily with saline; -treated group: gavaged daily with 65 mg/mL, and granules were dissolved in saline; cisplatin-treated group: injected intraperitoneally with cisplatin 4 mg/kg every 5 days, 6 mice per group. The subcutaneous tumor volume and mass changes of mice were measured, and the toxic effects of on heart, liver, spleen, lung and kidney as well as the therapeutic effects of were observed in the mice bearing tumor.

RESULTS

granules concentration-dependently inhibited the proliferation and survival of PC-9 and A549 cells, significantly promoted cell apoptosis, suppressed invasion and migration abilities of the cells, up-regulated the expression levels of E-cadherin and Bax, and down-regulated the expressions of Bcl-2, vimentin, MMP2, and MMP9. In the tumor-bearing mice, treatment with significantly inhibited tumor growth without producing obvious toxicity in the vital organs.

CONCLUSION

can inhibit proliferation, invasion, and migration and induces apoptosis of NSCLC cells .