Study on the expression of lncRNA in oral squamous cell carcinoma.

BACKGROUND

Oral squamous cell carcinoma (OSCC) is one of the most malignant tumors in the oral and maxillofacial region, with a poor prognosis. Previous studies have shown that long non-coding RNAs (lncRNAs) play a crucial role in tumor development by regulating the biological behavior of various cancer cells. The aim of this study is to explore the role and potential mechanisms of lncRNA in OSCC.

METHODS

Real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression levels of lncRNA in OSCC tissues and cell lines. Cell proliferation, migration and invasion were conducted to assess the biological functions of OSCC cell lines.

RESULTS

The expression of lncRNA in OSCC tissues was higher compared to that of adjacent non-cancerous tissues, and its expression level was associated with the depth of tumor infiltration, lymph node metastasis, and tumor node metastasis (TNM) staging. Compared to the control group of normal human oral keratinocytes (HOK), the expression of lncRNA was also elevated in OSCC cell lines. Knockdown of lncRNA significantly affected the proliferation, migration, and invasion ability of OSCC cells. However, when lncRNA was further overexpressed, changes in cell proliferation and migration ability did not show statistical differences.

CONCLUSIONS

LncRNA is highly expressed in OSCC, and its expression level is positively correlated with the depth of tumor infiltration, lymph node metastasis, and TNM staging. LncRNA is involved in regulating the proliferation, migration, and invasion of OSCC cells.