Wang Dongyong, Li Rui, Liu Qian, Deng Chao, Zhou Qiong, Wen Wenjie, Chai Lin
Oral Disease Research Center, School of Stomatology, Binjiang Campus, Wannan Medical College, Wuhu, China.
Anhui Province Engineering Research Center for Dental Materials and Application, Wuhu, China.
Transl Cancer Res. 2024 Oct 31;13(10):5202-5213. doi: 10.21037/tcr-24-467. Epub 2024 Oct 29.
Oral squamous cell carcinoma (OSCC) is one of the most malignant tumors in the oral and maxillofacial region, with a poor prognosis. Previous studies have shown that long non-coding RNAs (lncRNAs) play a crucial role in tumor development by regulating the biological behavior of various cancer cells. The aim of this study is to explore the role and potential mechanisms of lncRNA in OSCC.
Real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression levels of lncRNA in OSCC tissues and cell lines. Cell proliferation, migration and invasion were conducted to assess the biological functions of OSCC cell lines.
The expression of lncRNA in OSCC tissues was higher compared to that of adjacent non-cancerous tissues, and its expression level was associated with the depth of tumor infiltration, lymph node metastasis, and tumor node metastasis (TNM) staging. Compared to the control group of normal human oral keratinocytes (HOK), the expression of lncRNA was also elevated in OSCC cell lines. Knockdown of lncRNA significantly affected the proliferation, migration, and invasion ability of OSCC cells. However, when lncRNA was further overexpressed, changes in cell proliferation and migration ability did not show statistical differences.
LncRNA is highly expressed in OSCC, and its expression level is positively correlated with the depth of tumor infiltration, lymph node metastasis, and TNM staging. LncRNA is involved in regulating the proliferation, migration, and invasion of OSCC cells.
口腔鳞状细胞癌（OSCC）是口腔颌面部最恶性的肿瘤之一，预后较差。先前的研究表明，长链非编码RNA（lncRNAs）通过调节各种癌细胞的生物学行为在肿瘤发展中起关键作用。本研究的目的是探讨lncRNA在OSCC中的作用及潜在机制。
采用实时荧光定量聚合酶链反应（RT-qPCR）检测OSCC组织和细胞系中lncRNA的表达水平。进行细胞增殖、迁移和侵袭实验以评估OSCC细胞系的生物学功能。
与相邻非癌组织相比，lncRNA在OSCC组织中的表达更高，其表达水平与肿瘤浸润深度、淋巴结转移和肿瘤-淋巴结-转移（TNM）分期相关。与正常人口腔角质形成细胞（HOK）对照组相比，lncRNA在OSCC细胞系中的表达也升高。敲低lncRNA显著影响OSCC细胞的增殖、迁移和侵袭能力。然而，当lncRNA进一步过表达时，细胞增殖和迁移能力的变化未显示出统计学差异。
LncRNA在OSCC中高表达，其表达水平与肿瘤浸润深度、淋巴结转移和TNM分期呈正相关。LncRNA参与调节OSCC细胞的增殖、迁移和侵袭。
