Ⅰ型纤维中高度表达的肌肉因子脑源性神经营养因子抑制肌管向Ⅱ型纤维分化。
Myokine BDNF highly expressed in Type I fibers inhibits the differentiation of myotubes into Type II fibers.
机构信息
Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan.
Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan.
出版信息
Mol Biol Rep. 2024 Nov 12;51(1):1143. doi: 10.1007/s11033-024-10044-3.
BACKGROUND
Myofibers are broadly classified as slow-twitch (Type I) and fast-twitch (Type II) fibers. These two types of myofibers coexist within the same skeletal muscle tissue, determining the contractile and metabolic properties of skeletal muscle tissue by fiber type distribution.
METHODS AND RESULTS
By examining each fiber type separately, we confirmed that brain-derived neurotrophic factor (BDNF) gene is highly expressed in Type I fibers. When exposed to BDNF, primary myotubes exhibited reduced expression of Myosin Heavy Chain (MyHC) II, a marker protein characteristic of Type II fibers. BDNF overexpression in regenerating muscle tissue led to a decrease in the distribution of Type IIA fibers.
CONCLUSIONS
We suggest that BDNF highly expressed in Type I fibers downregulates MyHC II expression in myotubes, eventually inhibiting Type IIA fiber generation.
背景
肌纤维可广义地分为慢肌纤维（I 型）和快肌纤维（II 型）。这两种肌纤维存在于同一骨骼肌组织中，通过纤维类型分布决定骨骼肌组织的收缩和代谢特性。
方法和结果
通过分别检查每种纤维类型，我们证实脑源性神经营养因子（BDNF）基因在 I 型纤维中高度表达。当原代肌管暴露于 BDNF 时，II 型纤维的标志性蛋白肌球蛋白重链（MyHC）II 的表达减少。在再生肌肉组织中过表达 BDNF 导致 IIA 型纤维分布减少。
结论
我们认为 I 型纤维中高表达的 BDNF 下调肌管中 MyHC II 的表达，最终抑制 IIA 型纤维的生成。
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