Lab of Beef Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, PR China; National R&D Center for Beef Processing Technology, Tai'an, Shandong 271018, PR China.
Lab of Beef Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, PR China; National R&D Center for Beef Processing Technology, Tai'an, Shandong 271018, PR China.
Meat Sci. 2023 Oct;204:109287. doi: 10.1016/j.meatsci.2023.109287. Epub 2023 Jul 20.
The purpose of this study was to evaluate the impact of resveratrol on slow-twitch muscle fiber expression in bovine myotubes. The results revealed that resveratrol enhanced slow myosin heavy chain (MyHC) and suppressed fast MyHC protein expression, accompanied by increased MyHC I/IIa and decreased MyHC IIx/IIb mRNA levels in bovine myotubes (P < 0.05). Resveratrol also enhanced the activities of succinic dehydrogenase (SDH), malate dehydrogenase (MDH) and the mitochondrial DNA (mtDNA) content, but reduced lactate dehydrogenase (LDH) activity (P < 0.05). Meanwhile, the protein and gene expression of AMPK, SIRT1 and PGC-1α were upregulated by resveratrol (P < 0.05). Furthermore, PGC-1α inhibitor SR-18292 could attenuate resveratrol-induced muscle fiber conversion from fast-twitch to slow-twitch. These results suggest that resveratrol might promote muscle fiber type transition from fast-twitch to slow-twitch through the AMPK/PGC-1α signaling pathway and mitochondrial biogenesis in bovine myotubes.
本研究旨在评估白藜芦醇对牛肌管中慢肌纤维表达的影响。结果表明，白藜芦醇增强了慢肌肌球蛋白重链（MyHC）的表达，抑制了快肌 MyHC 蛋白的表达，同时伴随着牛肌管中 MyHC I/IIa 的增加和 MyHC IIx/IIb mRNA 水平的降低（P<0.05）。白藜芦醇还增强了琥珀酸脱氢酶（SDH）、苹果酸脱氢酶（MDH）和线粒体 DNA（mtDNA）的活性，但降低了乳酸脱氢酶（LDH）的活性（P<0.05）。同时，白藜芦醇上调了 AMPK、SIRT1 和 PGC-1α 的蛋白和基因表达（P<0.05）。此外，PGC-1α 抑制剂 SR-18292 可以减弱白藜芦醇诱导的牛肌管中快肌纤维向慢肌纤维的转化。这些结果表明，白藜芦醇可能通过 AMPK/PGC-1α 信号通路和线粒体生物发生促进牛肌管中肌肉纤维类型从快肌向慢肌的转变。
