Immunology and Microbiology Laboratory, Department of Zoology, University of North Bengal, Siliguri, West Bengal, India.
Department of Zoology, Siliguri College, Siliguri, West Bengal, India.
PLoS One. 2024 Nov 12;19(11):e0313548. doi: 10.1371/journal.pone.0313548. eCollection 2024.
Hepatic complications are the major health issues associated with dietary intake of calorie saturated food e.g. high-fat diet (HFD). Recent studies have revealed the beneficial effects of probiotics in HFD fed mice with hepatic complications. Some probiotic Lactic acid bacteria (LAB) e.g. Lactobacillus plantarum have drawn our attention in managing hepatic complications. Here, we aim to elucidate the protective effects of L. plantarum KAD strain, isolated from ethnic fermented food 'Kinema' in HFD-fed mice as, a preventive approach. Eighteen Swiss albino mice were equally divided into 3 groups: Normal Diet (ND), negative control (HFD), and HFD-fed with oral L. plantarum KAD supplementation (LP). All the experimental groups were subjected to specific diet according to grouping for eight weeks. After completion of the regime, subjects were anesthetized and sacrificed. Organs, blood, and fecal samples were collected and stored appropriately. Physical indices, including body weight gain, organ co-efficients were calculated along with assessment of glycemic, lipidomic, hepatic, oxidative stress, inflammatory, and histological parameters. Gut microbiota analysis was performed using 16s V3-V4 fecal metagenomic profiling, and sequencing were done using Illumina Miseq system. Oral administration of L. plantarum KAD is found to significantly (p<0.05) restore metabolic health by normalizing glycemic, lipidomic, hepatic parameters, oxidative stress and inflammatory parameters. Moreover, LP group (7.08±0.52 mg/g) showed significantly (p<0.001) decreased hepatic triglyceride level compared to HFD group (20.07±1.32 mg/g). L. plantarum KAD improved the adipocytic, and colonic histomorphology with significantly better scoring pattern. LP group (1.83±0.41) showed a significantly (p<0.001) reduced hepatic score compared to negative control group (5.00±0.63), showing reduced hepatosteatosis, and immune infiltration. The strain modulated gut health by altering its microbial composition positively towards normalization. In conclusion, the results of the experiment suggest that prophylactic L. plantarum KAD administration has beneficial effects on the onset of HFD induced hepatic complications in mice. Further studies are needed, on this strain for its clinical use as dietary supplement.
肝脏并发症是与高热量食物摄入相关的主要健康问题，例如高脂肪饮食（HFD）。最近的研究表明，益生菌对 HFD 喂养的伴有肝脏并发症的小鼠具有有益作用。一些益生菌乳酸菌（LAB），例如植物乳杆菌，已引起我们对管理肝脏并发症的关注。在这里，我们旨在阐明从民族发酵食品“Kinema”中分离出的植物乳杆菌 KAD 菌株对 HFD 喂养的小鼠的肝脏并发症的保护作用，作为一种预防方法。将 18 只瑞士白化小鼠平均分为 3 组：正常饮食（ND）、阴性对照（HFD）和 HFD 喂养加口服植物乳杆菌 KAD 补充剂（LP）。所有实验组均根据分组接受特定饮食 8 周。方案完成后，对实验对象进行麻醉并处死。收集和适当保存器官、血液和粪便样本。计算体重增加、器官系数等生理指标，并评估血糖、脂质组学、肝脏、氧化应激、炎症和组织学参数。使用 16s V3-V4 粪便宏基因组谱进行肠道微生物组分析，并使用 Illumina Miseq 系统进行测序。结果表明，口服植物乳杆菌 KAD 可通过使血糖、脂质组学、肝脏参数、氧化应激和炎症参数正常化，显著（p<0.05）恢复代谢健康。此外，LP 组（7.08±0.52mg/g）的肝甘油三酯水平显著（p<0.001）低于 HFD 组（20.07±1.32mg/g）。植物乳杆菌 KAD 改善了脂肪细胞和结肠的组织形态，其评分模式明显更好。LP 组（1.83±0.41）的肝评分显著（p<0.001）低于阴性对照组（5.00±0.63），表明肝脂肪变性和免疫浸润减少。该菌株通过积极改变其微生物组成来改善肠道健康，使其趋于正常化。总之，实验结果表明，预防性植物乳杆菌 KAD 给药对 HFD 诱导的小鼠肝脏并发症的发生具有有益作用。需要进一步研究该菌株作为膳食补充剂的临床用途。
