Gramma Vladislav, Olas Justyna Jadwiga, Zacharaki Vasiliki, Ponnu Jathish, Musialak-Lange Magdalena, Wahl Vanessa
Max Planck Institute of Molecular Plant Physiology, Department Metabolic Networks, 14476 Potsdam, Germany.
Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 90736 Umeå, Sweden.
Plant Physiol. 2024 Dec 23;197(1). doi: 10.1093/plphys/kiae594.
The timing of flowering in plants is modulated by both carbon (C) and nitrogen (N) signaling pathways. In a previous study, we established a pivotal role of the sucrose-signaling trehalose 6-phosphate pathway in regulating flowering under N-limited short-day conditions. In this work, we show that both wild-type Arabidopsis (Arabidopsis thaliana) plants grown under N-limited conditions and knock-down plants of TREHALOSE PHOSPHATE SYNTHASE 1 induce FLOWERING LOCUS C (FLC) expression, a well-known floral repressor associated with vernalization. When exposed to an extended period of cold, a flc mutant fails to respond to N availability and flowers at the same time under N-limited and full-nutrition conditions. Our data suggest that SUCROSE NON-FERMENTING 1 RELATED KINASE 1-dependent trehalose 6-phosphate-mediated C signaling and a mechanism downstream of N signaling (likely involving NIN-LIKE PROTEIN 7) impact the expression of FLC. Collectively, our data underscore the existence of a multi-factor regulatory system in which the C and N signaling pathways jointly govern the regulation of flowering in plants.
植物开花的时间受到碳（C）和氮（N）信号通路的调节。在之前的一项研究中，我们确立了蔗糖信号转导海藻糖6-磷酸途径在氮素有限的短日照条件下调控开花中的关键作用。在这项工作中，我们发现，在氮素有限条件下生长的野生型拟南芥植株以及海藻糖磷酸合酶1基因敲除植株均会诱导开花抑制因子开花位点C（FLC）的表达，FLC是一种与春化作用相关的著名开花抑制因子。当暴露于长时间低温时，flc突变体对氮素有效性无响应，在氮素有限和全营养条件下同时开花。我们的数据表明，蔗糖非发酵-1相关激酶1依赖性海藻糖6-磷酸介导的碳信号以及氮信号下游的一种机制（可能涉及类NIN蛋白7）影响FLC的表达。总体而言，我们的数据强调了存在一种多因素调控系统，其中碳和氮信号通路共同控制植物开花的调节。
