Yin Lingqian, Wang Xinyu, Zhang Donghao, Lin Zhongzhen, Wang Yan, Yu Chunlin, Jie Hang, Xu Feng, Yang Chaowu, Liu Yiping
State Key Laboratory of Swine and Poultry Breeding Industry, Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, and Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, China.
Poult Sci. 2024 Dec;103(12):104495. doi: 10.1016/j.psj.2024.104495. Epub 2024 Nov 3.
To deeply understanding the impact of peripheral energy level on the development of ovaries during the sexual maturation of chicken, in this study, the ovaries and serum of sexually mature and immature chickens at the same age from different energy level groups were collected, and the proteome and metabolome were detected. The results of ovarian and serum metabolomics revealed that dietary energy levels affected the energy metabolism and fatty acid oxidation of ovary in chicken, including the up-regulated expression of dihydroacetone phosphate and α-linolenic acid in high energy level groups. The results of proteomics showed that peripheral energy levels affected the catecholamine biosynthesis and metabolism in ovary before sexual maturation. The integrating analysis revealed that increased energy flux may influence ovarian development by regulating cholesterol reserves and steroid hormone synthesis in the ovaries. In vitro, the cultivation of chicken primary granulosa cells showed that sterol carrier protein 2 played a role in fatty acid synthesis and metabolism but did not significantly affect progesterone synthesis. Overall, dietary energy levels may be involved in the development of the ovaries during sexual maturation by influencing energy metabolism, biosynthesis of unsaturated fatty acids and steroid hormone within the ovaries.
为深入了解外周能量水平对鸡性成熟过程中卵巢发育的影响，本研究采集了不同能量水平组同龄性成熟和未成熟鸡的卵巢及血清，检测了蛋白质组和代谢组。卵巢和血清代谢组学结果显示，日粮能量水平影响鸡卵巢的能量代谢和脂肪酸氧化，包括高能水平组中磷酸二羟丙酮和α-亚麻酸表达上调。蛋白质组学结果表明，外周能量水平影响性成熟前卵巢中的儿茶酚胺生物合成和代谢。综合分析表明，能量通量增加可能通过调节卵巢中的胆固醇储备和类固醇激素合成来影响卵巢发育。体外培养鸡原代颗粒细胞表明，固醇载体蛋白2在脂肪酸合成和代谢中起作用，但对孕酮合成无显著影响。总体而言，日粮能量水平可能通过影响卵巢内的能量代谢、不饱和脂肪酸生物合成和类固醇激素参与鸡性成熟过程中卵巢的发育。
