Huang Xuan, Li Shibao, Yin Zhaozheng
Animal Science College, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
Animals (Basel). 2025 Mar 22;15(7):912. doi: 10.3390/ani15070912.
Egg production is a complex biological process closely linked to ovarian development and metabolic adaptation in laying hens. As the core reproductive organ, the ovary undergoes significant changes during different egg-laying stages. This study employed untargeted metabolomics to analyze metabolites in serum and ovarian tissues of hens at 20W and 30W. The results revealed that metabolic reprogramming in ovarian tissues was more pronounced than in serum. Shared metabolites between serum and ovarian tissues demonstrated coordinated interactions between systemic and local metabolic networks. The synthesis of prostaglandin E1 during lipid metabolism was identified as a key driver of ovulation and hormone production. Extracellular matrix remodeling and polyamine metabolism, particularly spermidine/spermine, enhanced cell adhesion and antioxidant capacity during ovarian development. These findings provide new insights into follicular development, ovulation regulation, and steroid hormone biosynthesis, while suggesting potential metabolic targets to improve poultry reproductive efficiency.
产蛋是一个复杂的生物学过程，与蛋鸡的卵巢发育和代谢适应密切相关。作为核心生殖器官，卵巢在不同产蛋阶段会发生显著变化。本研究采用非靶向代谢组学方法分析了20周龄和30周龄母鸡血清和卵巢组织中的代谢物。结果表明，卵巢组织中的代谢重编程比血清中更为明显。血清和卵巢组织之间的共享代谢物表明了全身和局部代谢网络之间的协同相互作用。脂质代谢过程中前列腺素E1的合成被确定为排卵和激素产生的关键驱动因素。细胞外基质重塑和多胺代谢，特别是亚精胺/精胺，在卵巢发育过程中增强了细胞粘附和抗氧化能力。这些发现为卵泡发育、排卵调节和类固醇激素生物合成提供了新的见解，同时也提出了提高家禽繁殖效率的潜在代谢靶点。
