Galletta Micaella, Combès Audrey, Mondello Luigi, Tranchida Peter Q, Pichon Valérie
Messina Institute of Technology C/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, Viale G. Palatucci Snc, 98168, Messina, Italy.
Department of Analytical, Bioanalytical Sciences, and Miniaturization (LSABM), UMR 8231 Chemistry, Biology and Innovation (CBI), ESPCI Paris, PSL University, Paris, France.
Anal Bioanal Chem. 2025 Jan;417(2):265-273. doi: 10.1007/s00216-024-05640-y. Epub 2024 Nov 12.
The aim of this work is the development of a biomimetic strategy involving a molecular recognition mechanism using aptamers immobilized on a solid support for the analysis of the mycotoxin zearalenone (ZEA) and two of its derivatives in human urine: alpha-zearelenol (α-ZEL) and beta-zearelenol (β-ZEL). Three oligonucleotide sequences reported in the literature as being specific to ZEA were thus covalently grafted onto activated sepharose, and a thorough study of the percolation and washing conditions was performed to promote the selective retention of the three targeted compounds. With the optimized extraction procedure, a strong and selective retention was obtained for ZEA and to a lesser extent α-ZEL and β-ZEL, with extraction recoveries of 88±9%, 77±15%, and 45±12% respectively, in standard solutions. Application of this procedure to spiked human urine strongly highlighted the efficiency of the clean-up effect resulting from the use of this selective sorbent. Limits of quantification of the whole analytical procedure including extraction on oligosorbent and LC-MS analysis were 0.18 and 0.24 ng mL, for ZEA and α-ZEL, respectively, thus demonstrating clearly the potential of the developed method for monitoring human dietary exposure to these compounds.
本研究旨在开发一种仿生策略，该策略涉及一种分子识别机制，利用固定在固体支持物上的适体来分析人尿中的霉菌毒素玉米赤霉烯酮（ZEA）及其两种衍生物：α-玉米赤霉醇（α-ZEL）和β-玉米赤霉醇（β-ZEL）。因此，将文献中报道的对ZEA具有特异性的三条寡核苷酸序列共价接枝到活化的琼脂糖上，并对渗滤和洗涤条件进行了深入研究，以促进三种目标化合物的选择性保留。采用优化的提取程序，在标准溶液中，ZEA以及程度稍低的α-ZEL和β-ZEL均获得了强烈且选择性的保留，提取回收率分别为88±9%、77±15%和45±12%。将该程序应用于加标的人尿中，突出显示了使用这种选择性吸附剂所产生的净化效果的效率。包括在寡核苷酸吸附剂上提取和LC-MS分析在内的整个分析程序的定量限，ZEA和α-ZEL分别为0.18和0.24 ng/mL，从而清楚地证明了所开发方法在监测人体饮食中这些化合物暴露方面的潜力。
