College of Animal Science and Technology, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Guangxi University, Nanning, 530004, Guangxi, China.
School of Materials Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, Jiangsu, China.
Mikrochim Acta. 2024 Nov 13;191(12):740. doi: 10.1007/s00604-024-06807-1.
Escherichia coli (E. coli) O157:H7 is an important food-borne pathogen that can cause hemorrhagic diarrhea and enteritis in humans and animals. Realizing the rapid quantitation of E. coli O157:H7 is of great significance for the guarantee of food safety and disease control. In this study, an electrochemical immunosensing technique based on a functionalized composite of Cu-metal organic framework (Cu-MOF) and poly (3,4-ethylenedioxythiophene)-poly (styrenesulfonate) (PEDOT:PSS) is developed, achieving rapid and sensitive quantitation of E. coli O157:H7 in food and clinical feces samples. The organic functionalization of Cu-MOF significantly improves the interface conductivity to facilitate electron transfer and provides the sulfonic groups (-SOH) to conjugate bio-recognizing elements for target determination. The immunosensor delivers a linear detection range of 3 × 10 ~ 3 × 10 cfu/mL, a low limit of detection (LOD) of 7.4 cfu/mL, and a short analysis time of 40 min. In addition, it does not show any cross-reactivity with other common pathogens and exhibits high repeatability with relative standard deviations (RSDs) all lower than 2.09%, providing a promising approach for warranting food safety and control of E. coli O157:H7 disease.
大肠杆菌（E. coli）O157：H7 是一种重要的食源性致病菌，可引起人类和动物的出血性腹泻和肠炎。实现大肠杆菌 O157：H7 的快速定量对于保证食品安全和疾病控制具有重要意义。在这项研究中，开发了一种基于功能化铜金属有机骨架（Cu-MOF）和聚（3,4-乙二氧基噻吩）-聚（苯乙烯磺酸盐）（PEDOT：PSS）的电化学生物传感技术，实现了对食品和临床粪便样本中大肠杆菌 O157：H7 的快速灵敏定量。Cu-MOF 的有机功能化显著提高了界面电导率，有利于电子转移，并提供了磺酸基（-SOH）用于目标测定的生物识别元素的结合。该免疫传感器提供了 3×10~3×10^cfu/mL 的线性检测范围，低检测限（LOD）为 7.4 cfu/mL，分析时间短至 40 分钟。此外，它与其他常见病原体没有任何交叉反应，并且具有高重复性，相对标准偏差（RSD）均低于 2.09%，为保证食品安全和控制大肠杆菌 O157：H7 疾病提供了一种有前途的方法。
