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采用非靶向代谢组学技术鉴定乙基丙酮酸给药后马血液代谢途径的变化。

Identification of altered blood metabolic pathways in equines following ethyl pyruvate administration using non-targeted metabolomics.

机构信息

Department of Pharmaceutical Engineering, Inje University, Gimhae, Republic of Korea.

Department of Animal Science, College of Natural Resource & Life Science, Pusan National University, Busan, Republic of Korea.

出版信息

Sci Rep. 2024 Nov 12;14(1):27684. doi: 10.1038/s41598-024-75734-1.

DOI:10.1038/s41598-024-75734-1
PMID:39532936
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11557697/
Abstract

Ethyl pyruvate (EP) has emerged as a promising compound with potential therapeutic benefits attributed to its anti-inflammatory and antioxidant properties. This study aimed to understand the effects of EP on plasma metabolites and immune cells in horses, utilizing advanced liquid chromatography-mass spectrometry (LC-MS)-based metabolomics, quantitative polymerase chain reaction (qPCR), and blood chemistry analyses. Our comprehensive analysis detected 2,366 ions, and 126 metabolites were accurately identified. Remarkably, EP administration induced significant changes in 28 metabolites at 1 h and 11 metabolites at 8 h, highlighting its time-dependent impact on metabolic pathways such as phenylalanine and arginine biosynthesis. Moreover, EP significantly lowered the expression of inflammatory markers interleukin (IL)-6 and heme oxygenase (HO)-1, indicating its potential as an anti-inflammatory agent. Blood chemistry analysis revealed notable reductions in glucose and triglyceride levels. These findings demonstrate that EP is a substance with potential effects on pathways associated with inflammation, oxidative stress, and metabolic processes.

摘要

丙酮酸乙酯 (EP) 作为一种有前途的化合物，因其具有抗炎和抗氧化特性而备受关注。本研究旨在利用先进的液相色谱-质谱 (LC-MS) 代谢组学、定量聚合酶链反应 (qPCR) 和血液化学分析，了解 EP 对马血浆代谢物和免疫细胞的影响。我们的综合分析检测到 2366 个离子，准确鉴定出 126 种代谢物。值得注意的是，EP 给药在 1 小时时引起 28 种代谢物和 8 小时时引起 11 种代谢物的显著变化，突出了其对苯丙氨酸和精氨酸生物合成等代谢途径的时间依赖性影响。此外，EP 显著降低了炎症标志物白细胞介素 (IL)-6 和血红素加氧酶 (HO)-1 的表达，表明其作为抗炎剂的潜力。血液化学分析显示葡萄糖和甘油三酯水平显著降低。这些发现表明，EP 是一种对与炎症、氧化应激和代谢过程相关的途径具有潜在影响的物质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e97/11557697/c5ad2ff81972/41598_2024_75734_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e97/11557697/39b8d0d8bb3a/41598_2024_75734_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e97/11557697/56b54988c3d7/41598_2024_75734_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e97/11557697/507c98332096/41598_2024_75734_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e97/11557697/c5ad2ff81972/41598_2024_75734_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e97/11557697/39b8d0d8bb3a/41598_2024_75734_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e97/11557697/5a2b44ed17f5/41598_2024_75734_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e97/11557697/42063fff8aad/41598_2024_75734_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e97/11557697/56b54988c3d7/41598_2024_75734_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e97/11557697/507c98332096/41598_2024_75734_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e97/11557697/c5ad2ff81972/41598_2024_75734_Fig6_HTML.jpg

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本文引用的文献

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Using MetaboAnalyst 5.0 for LC-HRMS spectra processing, multi-omics integration and covariate adjustment of global metabolomics data.使用 MetaboAnalyst 5.0 进行 LC-HRMS 光谱处理、多组学整合和全局代谢组学数据的协变量调整。
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Ethyl pyruvate, a versatile protector in inflammation and autoimmunity.
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Microbial metabolism of L-tyrosine protects against allergic airway inflammation.L-酪氨酸的微生物代谢可预防过敏性气道炎症。
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Lifestyle, Oxidative Stress, and Antioxidants: Back and Forth in the Pathophysiology of Chronic Diseases.生活方式、氧化应激与抗氧化剂：慢性病病理生理学中的反复作用
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