Department of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225, Pirassununga, 13635-900, SP, Brazil.
Department of Animal Sciences, College of Agriculture, Auburn University, Auburn, AL, 36849, USA.
Sci Rep. 2024 Nov 12;14(1):27681. doi: 10.1038/s41598-024-78965-4.
We investigated the long-term effects of prenatal nutrition on pre-slaughter Nelore bulls using integrative transcriptome and metabolome analyses of liver tissue. Three prenatal nutritional treatments were administered to 126 cows: NP (control, mineral supplementation only), PP (protein-energy supplementation in the third trimester), and FP (protein-energy supplementation throughout pregnancy). Liver samples from 22.5 ± 1-month-old bulls underwent RNA-Seq and targeted metabolomics. Weighted correlation network analysis (WGCNA) identified treatment-associated gene and metabolite co-expression modules, further analyzed using MetaboAnalyst 6.0 (metabolite over-representation analysis and transcriptome-metabolome integrative analysis) and Enrichr (gene over-representation analysis). We identified several significant gene and metabolite modules, as well as hub components associated with energy, protein and oxidative metabolism, regulatory mechanisms, epigenetics, and immune function. The NP transcriptome-metabolome analysis identified key pathways (aminoacyl t-RNA biosynthesis, gluconeogenesis, and PPAR signaling) and hub components (glutamic acid, SLC6A14). PP highlighted pathways (arginine and proline metabolism, TGF-beta signaling, glyoxylate and dicarboxylate metabolism) with arginine and ODC1 as hub components. This study highlights the significant impact of prenatal nutrition on the liver tissue of Nelore bulls, shedding light on critical metabolic pathways and hub components related to energy and protein metabolism, as well as immune system and epigenetics.
我们使用肝脏组织的综合转录组和代谢组分析研究了产前营养对预屠宰内罗尔公牛的长期影响。对 126 头母牛进行了三种产前营养处理：NP（对照，仅补充矿物质）、PP（妊娠后期补充蛋白质-能量）和 FP（整个孕期补充蛋白质-能量）。22.5±1 月龄公牛的肝脏样本进行了 RNA-Seq 和靶向代谢组学分析。加权相关网络分析（WGCNA）确定了与处理相关的基因和代谢物共表达模块，进一步使用 MetaboAnalyst 6.0（代谢物过表达分析和转录组-代谢组综合分析）和 Enrichr（基因过表达分析）进行分析。我们确定了几个与能量、蛋白质和氧化代谢、调节机制、表观遗传学和免疫功能相关的显著基因和代谢物模块以及枢纽成分。NP 的转录组-代谢组分析确定了关键途径（氨酰 t-RNA 生物合成、糖异生和 PPAR 信号）和枢纽成分（谷氨酸、SLC6A14）。PP 突出了途径（精氨酸和脯氨酸代谢、TGF-β 信号、乙醛酸和二羧酸代谢），其枢纽成分是精氨酸和 ODC1。这项研究强调了产前营养对内罗尔公牛肝脏组织的重大影响，揭示了与能量和蛋白质代谢以及免疫系统和表观遗传学相关的关键代谢途径和枢纽成分。
