Yang Guowu, Dai Rongfeng, Ma Xiaoming, Huang Chun, Ma Xiaoyong, Li Xinyi, La Yongfu, Dingkao Renqing, Renqing Ji, Guo Xian, Zhaxi Ta, Liang Chunnian
Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
College of Life Sciences and Engineering, Northwest Minzu University, Lanzhou 730106, China.
Animals (Basel). 2024 Jan 26;14(3):406. doi: 10.3390/ani14030406.
Jersey-yak is a hybrid offspring of Jersey cattle and yak (). Changing the feeding system of Jersey-yak can significantly improve its growth performance. In this study, tandem mass tag (TMT) proteomics technology was used to determine the differentially expressed proteins (DEPs) of the (LL) muscle of Jersey-yak fed different protein levels of diet. The results showed that compared with the traditional grazing feeding, the growth performance of Jersey-yaks was significantly improved by crude protein supplementation after grazing. A total of 3368 proteins were detected in these muscle samples, of which 3365 were quantified. A total of 434 DEPs were identified. Through analyses, it was found that some pathways related to muscle growth and development were significantly enriched, such as Rap1 signaling pathway, mTOR signaling pathway, and TGF-beta signaling pathway. A number of DEPs enriched in these pathways are related to muscle cell development, differentiation, and muscle development, including integrin subunit alpha 7 (ITGA7), myosin heavy chain 8 (MYH8), and collagen type XII alpha 1 chain (COL12A1). In conclusion, the results of this study provide insights into the proteomics of different feeding patterns of Jersey-yak, providing a stronger basis for further understanding the biological mechanism of hybrid varieties.
娟珊牦牛是娟珊牛和牦牛的杂交后代。改变娟珊牦牛的饲养方式可显著提高其生长性能。本研究采用串联质谱标签（TMT）蛋白质组学技术，测定了饲喂不同蛋白质水平日粮的娟珊牦牛腰大肌中差异表达蛋白（DEP）。结果表明，与传统放牧饲养相比，放牧后补充粗蛋白可显著提高娟珊牦牛的生长性能。在这些肌肉样本中共检测到3368种蛋白质，其中3365种被定量。共鉴定出434个差异表达蛋白。通过分析发现，一些与肌肉生长发育相关的通路显著富集，如Rap1信号通路、mTOR信号通路和TGF-β信号通路。这些通路中富集的许多差异表达蛋白与肌肉细胞发育、分化及肌肉发育有关，包括整合素α7亚基（ITGA7）、肌球蛋白重链8（MYH8）和Ⅻ型胶原α1链（COL12A1）。总之，本研究结果为娟珊牦牛不同饲养方式的蛋白质组学研究提供了见解，为进一步了解杂交品种的生物学机制提供了更有力的依据。
