School of Public Health and Preventive Medicine, Faculty of Medicine, Nursing, and Health Sciences, Monash University, Melbourne, Australia.
Faculty of Medicine and Health Sciences, Udayana University, Bali, Indonesia.
Sci Rep. 2024 Nov 12;14(1):27738. doi: 10.1038/s41598-024-79447-3.
The environmental realm has been acknowledged as a pivotal arena for the emergence and propagation of antimicrobial resistance. To further explore insight into antimicrobial resistance dynamics beyond clinical and veterinary settings, we embarked on an environmental surveillance initiative targeting the prevalence of antibiotic-resistant bacteria within the bustling confines of an international airport in Japan. Our findings illuminate a high prevalence of methicillin-resistant staphylococci (46.3%) on frequently contacted surfaces in the public domain. Notably, Staphylococcus haemolyticus and S. epidermidis emerged as the preeminent carriers of the mecA gene. Intriguingly, we encountered a virulent strain of livestock-associated MRSA harboring a PVL-positive ST1232 clone, CC398 lineage. Further scrutiny unveiled a repertoire of resistance mechanisms, the methicillin-resistant isolates exhibited two or more resistance genes conferring resistance against different types of antibiotics, including beta-lactams, macrolides, lincosamides, aminoglycosides, chloramphenicol, and fosfomycin. Revealing multidrug-resistant CoNS and a LA-MRSA across various surfaces in urban public areas unearths a looming public health hazard. Thus, implementation of molecular surveillance is imperative, augmenting our capacity for early detection and mitigation of the insidious spread and potential transfer of antibiotic resistance genes and virulence factors amidst urban settings, notably within pivotal nodes such as airports.
环境领域已被公认为是抗菌药物耐药性出现和传播的关键领域。为了进一步深入了解临床和兽医环境以外的抗菌药物耐药性动态，我们在日本一个国际机场开展了一项环境监测计划，旨在调查机场内抗生素耐药菌的流行情况。我们的研究结果表明，在公众经常接触的表面，耐甲氧西林葡萄球菌（MRSA）的检出率很高（46.3%）。值得注意的是，表皮葡萄球菌和溶血葡萄球菌是 mecA 基因的主要携带菌。有趣的是，我们发现了一株携带 PVL 阳性 ST1232 克隆、CC398 谱系的高毒力的牲畜相关 MRSA 菌株。进一步研究揭示了一系列耐药机制，耐甲氧西林的分离株表现出两种或更多种耐药基因，对不同类型的抗生素具有耐药性，包括β-内酰胺类、大环内酯类、林可酰胺类、氨基糖苷类、氯霉素和磷霉素。在城市公共区域的各种表面发现了耐多药凝固酶阴性葡萄球菌（CoNS）和社区获得性耐甲氧西林金黄色葡萄球菌（LA-MRSA），这揭示了一个潜在的公共卫生危害。因此，实施分子监测至关重要，可以增强我们在城市环境中（特别是在机场等重要节点）早期检测和减轻抗菌药物耐药基因和毒力因子隐匿传播和潜在转移的能力。
