Rastegar-Moghaddam Seyed Hamidreza, Akbarian Mahsan, Rajabian Arezoo, Alipour Fatemeh, Hojjati Shargh AmirHossein, Masoomi Reza, Ebrahimzadeh Bideskan Alireza, Hosseini Mahmoud
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Naunyn Schmiedebergs Arch Pharmacol. 2025 May;398(5):5237-5248. doi: 10.1007/s00210-024-03593-8. Epub 2024 Nov 13.
There are several interactions between thyroid hormones (THs) and kidney and heart function. Consequently, THs deficit results in profound changes in renal and cardiac function regulation. Interestingly, emerging evidence suggests that vitamin D (Vit D) may benefit to fibrotic lesions in various tissues. Herein, this study was designed to investigate the potential impact of Vit D on renal and cardiac fibrosis in hypothyroid rats. Forty male Wistar rats were divided into four groups as follow: control, hypothyroid (0.05% PTU in drinking water), and hypothyroid + Vit D (PTU and doses of 100 or 500 IU/kg/day, by gavage) groups. After 6 weeks, biochemical parameters such as creatinine and urea in serum samples, and oxidative stress markers including malondialdehyde (MDA), total thiol groups, and superoxide dismutase (SOD) in renal and cardiac tissues homogenate were measured. Also, renal and cardiac fibrosis was evaluated histologically using Masson's trichrome staining. Hypothyroidism significantly increased creatinine and urea. Also, in hypothyroid group renal and cardiac fibrosis as well as MDA were increased, while anti-oxidative markers including total thiol group and SOD were decreased. Administration of Vit D significantly improved these alterations in oxidative stress markers and fibrosis in renal and cardiac tissues. In conclusion, this study highlighted that Vit D supplementation reduced renal and cardiac fibrosis and improved oxidative stress. These results support the emerging experimental findings linking Vit D being introduced as a potential therapeutic agent.
甲状腺激素（THs）与肾脏和心脏功能之间存在多种相互作用。因此，THs缺乏会导致肾脏和心脏功能调节发生深刻变化。有趣的是，新出现的证据表明维生素D（Vit D）可能对各种组织中的纤维化病变有益。在此，本研究旨在探讨Vit D对甲状腺功能减退大鼠肾脏和心脏纤维化的潜在影响。将40只雄性Wistar大鼠分为四组如下：对照组、甲状腺功能减退组（饮用水中含0.05%丙硫氧嘧啶）和甲状腺功能减退+Vit D组（丙硫氧嘧啶，通过灌胃给予100或500 IU/kg/天的剂量）。6周后，测量血清样本中的肌酐和尿素等生化参数，以及肾脏和心脏组织匀浆中的氧化应激标志物，包括丙二醛（MDA）、总巯基和超氧化物歧化酶（SOD）。此外，使用Masson三色染色法对肾脏和心脏纤维化进行组织学评估。甲状腺功能减退显著增加了肌酐和尿素。此外，在甲状腺功能减退组中，肾脏和心脏纤维化以及MDA增加，而包括总巯基和SOD在内的抗氧化标志物减少。给予Vit D显著改善了肾脏和心脏组织中氧化应激标志物和纤维化的这些改变。总之，本研究强调补充Vit D可减少肾脏和心脏纤维化并改善氧化应激。这些结果支持了将Vit D作为一种潜在治疗剂的新出现的实验发现。
