巴西马拉尼昂州一家儿科肿瘤病房里光滑念珠菌的克隆性暴发。

Clonal outbreak of Candida vulturna in a paediatric oncology ward in Maranhão, Brazil.

作者信息

de Macedo Alessandra Teixeira, Santos Daniel Wagner de Castro Lima, Spruijtenburg Bram, de Souza Dayse Azevedo Coelho, Dos Santos Barbosa Leila Ferreira Moreira, Marques Sirlei Garcia, Dos Santos Julliana Ribeiro Alves, Meijer Eelco F J, de Groot Theun, de Azevedo Conceição de Maria Pedrozo E Silva, Meis Jacques F

机构信息

Universidade Federal do Maranhão (UFMA)-Programa de Pós Graduação em Ciências da Saúde, São Luís, MA, Brazil.

Instituto D'Or de Pesquisa e Ensino (IDOR), São Luís, MA, Brazil; Hospital Universitário, Universidade Federal do Maranhão, São Luís, MA, Brazil.

出版信息

J Infect. 2024 Dec;89(6):106349. doi: 10.1016/j.jinf.2024.106349. Epub 2024 Nov 12.

DOI:10.1016/j.jinf.2024.106349

PMID:39537034

Abstract

OBJECTIVE

To describe an outbreak due to Candida vulturna, a newly emerging Candida species belonging to the Candida haemulonii species complex in the Metschnikowiaceae family.

METHODS

In this retrospective cohort study we genotyped 14 C. vulturna bloodstream isolates, occurring in a 4-month-period in paediatric cancer patients in a Brazilian hospital. To prove an outbreak, ITS sequence analysis and whole genome sequencing (WGS) was done. Antifungal susceptibility was performed with the reference CLSI method and the commercial Sensititre YeastOne (SYO) YO10 plates. A control C. vulturna isolate from another region in Brazil was included in all analyses.

RESULTS

MALDI-TOF-MS identified isolates as C. pseudohaemulonii and C. duobushaemulonii albeit with low scores and therefore molecular methods were required for accurate identification. ITS sequence analyses clearly differentiated C. vulturna from other species in the C. haemulonii species complex. WGS proved the presence of a clonal outbreak with C. vulturna involving 14 paediatric patients. Antifungal susceptibility testing (AFST) with two methods showed the isolates had low MICs of commonly available antifungals.

CONCLUSION

This study describes an outbreak due to the rare yeast C. vulturna, related to C. auris, during a four-month period in patients admitted to a paediatric oncology ward in a Brazilian hospital. In contrast to previous studies the yeast was susceptible to all antifungals and patient outcome was good.

摘要

目的

描述一起由伏尔图纳念珠菌引起的暴发，该菌是梅奇尼科夫酵母科中一个新出现的念珠菌物种，属于哈氏念珠菌复合种。

方法

在这项回顾性队列研究中，我们对巴西一家医院儿科癌症患者在4个月内分离出的14株伏尔图纳念珠菌血流感染菌株进行了基因分型。为证实暴发情况，进行了ITS序列分析和全基因组测序（WGS）。采用参考CLSI方法和商业Sensititre YeastOne（SYO）YO10平板进行抗真菌药敏试验。所有分析均纳入了来自巴西另一个地区的一株伏尔图纳念珠菌对照菌株。

结果

基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）将分离株鉴定为拟哈氏念珠菌和多布哈氏念珠菌，尽管得分较低，因此需要分子方法进行准确鉴定。ITS序列分析清楚地将伏尔图纳念珠菌与哈氏念珠菌复合种中的其他物种区分开来。WGS证明存在一起由伏尔图纳念珠菌引起的克隆性暴发，涉及14名儿科患者。两种方法的抗真菌药敏试验（AFST）显示，分离株对常用抗真菌药物的最低抑菌浓度较低。