LINC00470 促进睾丸生殖细胞肿瘤的恶性进展。
LINC00470 promotes malignant progression of testicular germ cell tumors.
机构信息
Department of Urology, Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
Department of Blood Transfusion, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.
出版信息
Mol Biol Rep. 2024 Nov 14;51(1):1152. doi: 10.1007/s11033-024-10083-w.
BACKGROUND
Testicular germ cell tumor (TGCT) is a common malignant tumor in adolescents. Now, many long non-coding RNAs (LncRNAs) have been found to have an important function in TGCT. LINC00470 is specifically and highly expressed in TGCT, however, there is still no definite information concerning its role and underlying mechanism in TGCT. The purpose of this research was to look into the involvement of LINC00470 in TGCT and its intrinsic mechanism.
METHODS AND RESULTS
UCSC and GEPIA2 databases were used to analyze the expression of LINC00470, and the BEST website was used to perform GSEA enrichment analysis, immune infiltration analysis, and drug susceptibility analysis. SiRNA transfection was used to silence LINC00470 in TCAM-2 and NCCIT cells. Clone formation and Transwell assays were performed in TGCT cells to confirm the effects of LINC00470 on clone formation, migration, and invasion. Western Blot was performed to determine the expression of proteins related to the EMT and AKT signaling pathways. LINC00470 was specifically highly expressed in TGCT, and played a role in promoting tumor cell clone formation and cell metastasis by affecting the TGF-β and PI3K-AKT-mTOR signaling pathways to regulate the epithelial-mesenchymal transition (EMT) process; LINC00470 may also play a pro-tumor role by negatively regulating immune infiltration; in addition, the expression of LINC00470 was negatively correlated with the chemosensitivity of cisplatin in TGCT patients.
CONCLUSIONS
LINC00470 may play a significant role in the etiology and metastasis of TGCT through EMT and AKT-mediated signaling pathways.
背景
睾丸生殖细胞肿瘤（TGCT）是青少年中常见的恶性肿瘤。现在，已经发现许多长非编码 RNA（LncRNA）在 TGCT 中具有重要功能。LINC00470 在 TGCT 中特异性和高表达，然而，其在 TGCT 中的作用及其潜在机制仍没有明确的信息。本研究旨在探讨 LINC00470 参与 TGCT 的情况及其内在机制。
方法和结果
使用 UCSC 和 GEPIA2 数据库分析 LINC00470 的表达，使用 BEST 网站进行 GSEA 富集分析、免疫浸润分析和药物敏感性分析。使用 siRNA 转染沉默 TCAM-2 和 NCCIT 细胞中的 LINC00470。在 TGCT 细胞中进行克隆形成和 Transwell 实验，以验证 LINC00470 对克隆形成、迁移和侵袭的影响。Western Blot 用于确定与 EMT 和 AKT 信号通路相关的蛋白表达。LINC00470 在 TGCT 中特异性高表达，通过影响 TGF-β和 PI3K-AKT-mTOR 信号通路调节上皮-间充质转化（EMT）过程，从而促进肿瘤细胞克隆形成和细胞转移；LINC00470 还可能通过负向调节免疫浸润发挥促肿瘤作用；此外，LINC00470 的表达与 TGCT 患者顺铂化疗敏感性呈负相关。
结论
LINC00470 可能通过 EMT 和 AKT 介导的信号通路在 TGCT 的发病机制和转移中发挥重要作用。
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