regulates the metastasis of testicular germ cell tumors through epithelial-mesenchyme transition and immune pathways.

Testicular germ cell tumor (TGCT) is a relatively rare entity tumor, accounting for only 1% of all male cancers. However, it is the most common solid tumor in young men between 15 and 34 years old. Long noncoding RNAs (lncRNAs) are involved in various physiological and pathological processes. However, the functions of lncRNAs in TGCT have only rarely been investigated. LncRNAs associated with TGCT were identified using Gene Expression Omnibus (GEO) database and UCSC XENA database data mining. The effects of on NCCIT cell migration and invasion were evaluated in transwell assays. The expression levels of epithelial-mesenchyme transition (EMT)-related proteins in cells knockdown of were analyzed by Western blot. Correlation analyses between lncRNA expression and copy number variation (CNV) and immune cell infiltration were carried out using The Cancer Genome Atl as (TCGA) data. The effect of Panobinostatin targeting in TGCT cells was investigated. We observed higher expression in TGCT. The migratory and invasive properties of TGCT cells were augmented by , likely via its effects on modulating the expression of epithelial-mesenchyme transition (EMT) related proteins: CTNNB1, ZEB1, CDH2, Snail and VIM. Moreover, expression and CNV correlated negatively with the infiltration of immune cells. In addition, Panobinostat might be a possible candidate drug to target in TGCT. performs important pro-migration and invasion functions in the pathogenesis of TGCT. could be used as diagnostic, prognostic, immune marker and therapeutic target to develop effective treatment of TGCT.