Yang Zhifei, Zhang Jiaxu, Wang Chen, Yu Fangzheng, Yu Wen, Zhao Zheng
Sanya Science and Education Innovation Park, Wuhan University of Technology, Sanya 572000, China.
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China.
Biomater Sci. 2024 Dec 17;13(1):275-286. doi: 10.1039/d4bm01097c.
The healing of complex diabetic wounds with a hyperglycemic microenvironment and bacterial infection is considered an important clinical issue. In this study, glucose oxidase (GOx) and gold nanoclusters (AuNCs) were encapsulated in quaternary carboxymethyl chitosan (QCMCS)/sodium alginate oxide (OSA) hydrogels and were immersed in tannic acid (TA) solution to achieve antioxidant, antibacterial, pro-angiogenesis, pro-collagen deposition and real-time monitoring functions. studies showed that TA-QCMCS/OSA@GOx@AuNC hydrogels had inhibition rates of 98.99% and 99.99% against and , respectively, and the survival rate of mouse fibroblasts (L929) was over 95%. studies showed that TA-QCMCS/OSA@GOx@AuNC hydrogels were 97.28% effective in healing diabetic wounds. In addition, image signals from TA-QCMCS/OSA@GOx@AuNC hydrogels can be collected in real time to accurately obtain glucose concentration values of diabetic wounds and reflect the healing status of diabetic wounds in a timely manner. The results showed that TA-QCMCS/OSA@GOx@AuNC hydrogels provide a novel idea for real-time monitoring of diabetic wound treatment.
具有高血糖微环境和细菌感染的复杂糖尿病伤口的愈合被认为是一个重要的临床问题。在本研究中，葡萄糖氧化酶（GOx）和金纳米簇（AuNCs）被封装在季铵化羧甲基壳聚糖（QCMCS）/氧化海藻酸钠（OSA）水凝胶中，并浸入单宁酸（TA）溶液中，以实现抗氧化、抗菌、促血管生成、促胶原蛋白沉积和实时监测功能。研究表明，TA-QCMCS/OSA@GOx@AuNC水凝胶对金黄色葡萄球菌和大肠杆菌的抑制率分别为98.99%和99.99%，小鼠成纤维细胞（L929）的存活率超过95%。研究表明，TA-QCMCS/OSA@GOx@AuNC水凝胶在治疗糖尿病伤口方面的有效率为97.28%。此外，TA-QCMCS/OSA@GOx@AuNC水凝胶的图像信号可以实时采集，以准确获得糖尿病伤口的葡萄糖浓度值，并及时反映糖尿病伤口的愈合状态。结果表明，TA-QCMCS/OSA@GOx@AuNC水凝胶为糖尿病伤口治疗的实时监测提供了新思路。
