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蛋白质组学和泛素组学分析表明，微重力通过重塑非降解泛素化来影响小鼠心脏的葡萄糖代谢。

Proteomic and ubiquitinome analysis reveal that microgravity affects glucose metabolism of mouse hearts by remodeling non-degradative ubiquitination.

机构信息

State Key Laboratory of Proteomics, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China.

Institute of Microbiology (Chinese Academy of Sciences), CAS Key Laboratory of Pathogenic Microbiology and Immunology, Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China.

出版信息

PLoS One. 2024 Nov 14;19(11):e0313519. doi: 10.1371/journal.pone.0313519. eCollection 2024.

DOI:10.1371/journal.pone.0313519
PMID:39541295
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11563481/
Abstract

Long-term exposure to a microgravity environment leads to structural and functional changes in hearts of astronauts. Although several studies have reported mechanisms of cardiac damage under microgravity conditions, comprehensive research on changes at the protein level in these hearts is still lacking. In this study, proteomic analysis of microgravity-exposed hearts identified 156 differentially expressed proteins, and ubiquitinomic analysis of these hearts identified 169 proteins with differential ubiquitination modifications. Integrated ubiquitinomic and proteomic analysis revealed that differential proteomic changes caused by transcription affect the immune response in microgravity-exposed hearts. Additionally, changes in ubiquitination modifications under microgravity conditions excessively activated certain kinases, such as hexokinase and phosphofructokinase, leading to cardiac metabolic disorders. These findings provide new insights into the mechanisms of cardiac damage under microgravity conditions.

摘要

长期暴露在微重力环境下会导致宇航员心脏的结构和功能发生变化。虽然已有几项研究报道了微重力条件下心脏损伤的机制，但对这些心脏在蛋白质水平上的变化仍缺乏全面的研究。在这项研究中，对微重力暴露的心脏进行蛋白质组学分析，鉴定出 156 个差异表达的蛋白质，对这些心脏进行泛素组学分析，鉴定出 169 个具有差异泛素化修饰的蛋白质。泛素组学和蛋白质组学的综合分析表明，转录引起的差异蛋白质组变化会影响微重力暴露心脏的免疫反应。此外，微重力条件下泛素化修饰的变化会过度激活某些激酶，如己糖激酶和磷酸果糖激酶，导致心脏代谢紊乱。这些发现为微重力条件下心脏损伤的机制提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e30/11563481/c6350eb8b359/pone.0313519.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e30/11563481/ba4e477b51b8/pone.0313519.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e30/11563481/2b2c94dbc2a1/pone.0313519.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e30/11563481/2f62cfc9b6e0/pone.0313519.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e30/11563481/c6350eb8b359/pone.0313519.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e30/11563481/ba4e477b51b8/pone.0313519.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e30/11563481/2b2c94dbc2a1/pone.0313519.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e30/11563481/2f62cfc9b6e0/pone.0313519.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e30/11563481/c6350eb8b359/pone.0313519.g004.jpg

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