Department of Plant Pathology, University of Florida, Gainesville, FL 32611, USA.
Department of Plant Pathology, University of Florida, Gainesville, FL 32611, USA; Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA.
Ecotoxicol Environ Saf. 2024 Nov 15;287:117325. doi: 10.1016/j.ecoenv.2024.117325. Epub 2024 Nov 13.
Streptomycin (Str) and oxytetracycline (Otc) are widely used antibiotics to manage bacterial diseases in citrus and other crops. However, their impacts on the rhizosphere bacterial assembly and plant physiology are poorly understood. The aim of this study was to examine the effects of Str and Otc on the physiology (assimilation, transpiration rate, intracellular CO, and stomatal conductance to water vapor), rhizosphere bacterial assemblages (16S rRNA gene high-throughput amplicon sequencing), and rhizosphere metabolite profiles in healthy Citrus reticulata trees. The results indicated a reduction in photosynthesis after Str and Otc treatments, whereas CO outflow stayed constant. Both antibiotics decreased the culturable numbers of bacteria. Analysis of the microbiome showed changes in relative abundance of bacterial groups, specifically Pseudomonas, Agrobacterium, and Streptomyces, in response to the antibiotics. Metabolite profiles changed in streptomycin- and oxytetracycline-treated citrus plants suggesting response to microbe targets or induction of stress responses. This study advances knowledge of antibiotic-driven effects on the rhizosphere microbiome, rhizosphere metabolome, and plant physiology, which is essential for managing plant diseases while safeguarding rhizosphere ecology and long-term plant health.
链霉素（Str）和土霉素（Otc）被广泛用于治疗柑橘和其他作物的细菌性疾病。然而，它们对根际细菌组成和植物生理学的影响知之甚少。本研究旨在研究 Str 和 Otc 对健康柑橘树的生理（同化、蒸腾速率、细胞内 CO2 和水蒸气的气孔导度）、根际细菌组成（16S rRNA 基因高通量扩增子测序）和根际代谢物谱的影响。结果表明，Str 和 Otc 处理后光合作用减少，而 CO 流出保持不变。两种抗生素都减少了细菌的可培养数量。微生物组分析显示，细菌群体的相对丰度发生了变化，特别是 Pseudomonas、Agrobacterium 和 Streptomyces，对这些抗生素有反应。代谢物图谱在链霉素和土霉素处理的柑橘植物中发生了变化，表明对微生物靶标或诱导应激反应的响应。本研究增进了对抗生素对根际微生物组、根际代谢组和植物生理学的驱动效应的认识，这对于在保护根际生态和长期植物健康的同时管理植物病害至关重要。
