Green synthesis of silver nanoparticles from supercritical CO mediated Lagerstroemia speciosa extract: Characterization, antimicrobial and antibiofilm activity.

In the current study, optimal supercritical fluid extract (SFE) of Lagerstroemia speciosa (LS) leaves at pressure 29.59 MPa (MPa), temperature 89.50 °C and extraction time 53.85 min was used to extract phenolic compounds for the synthesis of silver nanoparticles (AgNPs). The synthesis was studied for 0-20 h. Initially the synthesis of nanoparticles (SFELS-AgNPs) was confirmed using UV -spectroscopy. It demonstrated a maximum surface plasmon resonance at 430 nm. The crystallite dimension of nanoparticles was determined using X-ray diffraction (XRD) (13.47 nm), Transmission electron microscopy (TEM), zeta potential analysis and energy-dispersive X-ray analysis (EDAX) were used to analyze the morphology, surface charge and presence of differential elements in SFELS-AgNPs respectively. Developed nanoparticles revealed antimicrobial activity against 2 g-positive viz. Staphylococcus aureus and Bacillus cereus, and 3 g-negative bacteria viz. Klebsiella pneumonia, Pseudomonas aeruginosa and Escherichia coli. The nanoparticle showed a minimum inhibitory concentration (MIC) of 64 μg/ml whereas the minimum bactericidal concentration (MBC) 128 μg/ml against K. pneumonia. They significantly inhibited K. pneumonia biofilm formation which was confirmed using scanning electron microscopy (SEM). The results were encouraging compared to the standards drug Chloramphenicol and other controls. The generated nanoparticles have highly effective antimicrobial properties against pathogenic bacteria.