Kumari Surbhi, Rohilla Rishika, Rani Sonia, Prabhakar Nirmal
Department of Biochemistry, Panjab University Chandigarh 160014 India
RSC Adv. 2025 Jun 25;15(27):21528-21540. doi: 10.1039/d5ra03037d. eCollection 2025 Jun 23.
This study focuses on the green synthesis of Ag-Au nanoparticles using extract and their potential application in electrochemical detection and estimation of ampicillin in raw milk. The synthesised nanoparticles were characterized using both physical as well as electrochemical techniques to assess their structural, morphological and conductive properties. The Ag-Au nanoparticles were covalently bonded to the hydroxylated fluorine doped tin oxide electrode surface through silane chemistry enabling the effective interaction of the 5' thiolated-aptamer with the nanoparticle-modified surface for aptasensor development. The proposed aptasensor displayed a highly sensitive and selective electrochemical response towards ampicillin within a range of 0.1 ng mL to 1000 ng mL with a limit of detection of 0.40 ng mL and a shelf-life of 30 days. Moreover, the aptasensor demonstrated reliable performance with both spiked milk samples and real milk samples, detecting a concentration of 194 ng mL in raw milk with results closely matching with those obtained by LC-MS. Therefore, this study highlights the feasibility of plant-based nanoparticle synthesis for developing cost-effective and efficient electrochemical sensors in food safety monitoring.
本研究聚焦于利用提取物绿色合成银-金纳米粒子及其在生乳中氨苄青霉素电化学检测与定量分析中的潜在应用。采用物理和电化学技术对合成的纳米粒子进行表征，以评估其结构、形态和导电性能。通过硅烷化学将银-金纳米粒子共价键合到羟基化氟掺杂氧化锡电极表面，从而使5'硫醇化适配体与纳米粒子修饰表面有效相互作用，用于开发适配体传感器。所提出的适配体传感器在0.1 ng/mL至1000 ng/mL范围内对氨苄青霉素表现出高度灵敏且选择性的电化学响应，检测限为0.40 ng/mL，保质期为30天。此外，该适配体传感器在加标牛奶样品和实际牛奶样品中均表现出可靠的性能，检测出生乳中浓度为194 ng/mL，结果与液相色谱-质谱联用仪所得结果紧密匹配。因此，本研究突出了基于植物的纳米粒子合成在食品安全监测中开发经济高效的电化学传感器的可行性。
