Department of Myxoma, Aerospace Center Hospital, Beijing, 100049, China.
Space Medical Center, Aerospace Center Hospital, Beijing, 100049, China.
J Transl Med. 2024 Nov 14;22(1):1022. doi: 10.1186/s12967-024-05730-6.
Pseudomyxoma peritonei (PMP) is a rare malignant peritoneal tumor that readily recurs and metastasizes. Studies have shown that cancer stem cells (CSCs) play an important role in tumor recurrence, metastasis, and prognosis.
In this study, our aim was to isolate CSCs from various tissues of PMP patients and compare their proliferation, migration, and anti-inflammatory abilities.
We identified CSCs subsets with markers CD133, CD166, and CD133/CD166 at the gene level using single-cell mRNA sequencing (scRNA-seq). Appendiceal CSCs (AC), peritoneal CSCs (PC), and mucous CSCs (MC) were obtained using MACSQuant Tyto sorting technology and FlowSight imaging flow cytometry. The cells were cultured and markers were identified. Finally, the functional phenotypes of the three cell types were compared.
CSCs content was highest in the appendiceal tumor tissue and lowest in the mucous tissue. The cell viability rate of the sorted CSCs was above 98%, and the positive rate of CD133 and CD166 was 70-80%, and CD133/CD166 was about 30%. Among the three types of CSCs, MC had the highest proliferation ability, and TNF-α has the greatest inhibitory effect on AC migration.
AC in patients was more inert and anti-inflammatory, whereas abdominal cavity MC and PC were more active. This study revealed the biological characteristics of CSCs in different tumor tissues of patients with PMP, providing a reference for future targeted CSCs therapy.
假性黏液瘤（PMP）是一种罕见的恶性腹膜肿瘤，易复发和转移。研究表明，癌症干细胞（CSC）在肿瘤复发、转移和预后中起着重要作用。
本研究旨在从 PMP 患者的不同组织中分离 CSC，并比较它们的增殖、迁移和抗炎能力。
我们使用单细胞 mRNA 测序（scRNA-seq）在基因水平上鉴定出 CSC 亚群，其标志物为 CD133、CD166 和 CD133/CD166。使用 MACSQuant Tyto 分选技术和 FlowSight 成像流式细胞术获得阑尾 CSC（AC）、腹膜 CSC（PC）和黏液 CSC（MC）。对细胞进行培养和标记，并最终比较三种细胞类型的功能表型。
CSC 含量在阑尾肿瘤组织中最高，在黏液组织中最低。分选的 CSC 细胞活力率高于 98%，CD133 和 CD166 的阳性率为 70-80%，CD133/CD166 约为 30%。在三种 CSC 中，MC 的增殖能力最强，TNF-α 对 AC 迁移的抑制作用最大。
与腹腔 MC 和 PC 相比，PMP 患者的 AC 更惰性和抗炎，而腹腔 MC 和 PC 更活跃。本研究揭示了 PMP 患者不同肿瘤组织中 CSC 的生物学特性，为未来靶向 CSC 治疗提供了参考。
