Cárdenas Rocio, Cabrera Olga L, Carrillo Maria A, Pineda Alejandra, Ahumada Martha L, Yañez Yohana, Ismail Hanafy, Paine Mark, Rivera Tatiana, Kroeger Axel
Faculty of Medicine, Centre for Medicine and Society, Master Programme Global Urban Health, Albert-Ludwigs-University Freiburg, Freiburg, Germany.
Instituto JWT, Universidad de los Andes, Trujillo, Venezuela.
Med Vet Entomol. 2025 Jun;39(2):252-265. doi: 10.1111/mve.12776. Epub 2024 Nov 14.
Ground water tanks are known to be preferred Aedes aegypti oviposition places providing opportunities for adult and larvae control. Therefore, a dual-effect insecticidal coating (IC) (alphacypermethrin/ pyriproxyfen) with a slow-release mechanism and safe for users could be applied within Aedes spp. breeding sites, representing a promising option. Bioassays were designed to determine the mortality and sterilizing effects on gravid mosquitoes exposed to IC. The effect of inhibition of emergence was evaluated in eggs, larvae and pupae exposed in different containers. For the water safety assessment concentrations of active ingredients were determined by reverse phase high performance liquid chromatography (RP-HPLC) and the health risk was calculated. The IC applied to the interior walls of water-holding containers showed efficacy against Ae. aegypti in terms of high gravid-female mortality (81% at 24 h, p < 0.01), sterilizing effect (inhibition of oviposition by 63%, p < 0.01) and emergence inhibition (100% in eggs, L3 and L4; 97% in pupae). The offspring rate was reduced [only 0.15 (38/250) new adults emerged per exposed gravid females as against 11.90 per unexposed female (2976/250) at baseline]. Emergence inhibition was recorded up to 12 months and adult mortality >80% up to 6 months. The use of water stored in treated containers, either for washing or drinking, is not expected to pose a health risk to users. IC applied to domestic water containers has dual and complementary action that reduces Ae. aegypti densities (immature and adult stages). This represents baseline information for a cluster randomized efficacy trial in Colombia.
众所周知，地下水罐是埃及伊蚊偏爱的产卵场所，为控制成蚊和幼虫提供了机会。因此，一种具有缓释机制且对使用者安全的双重作用杀虫涂层（IC）（高效氯氰菊酯/吡丙醚）可应用于伊蚊属的繁殖地，这是一个很有前景的选择。设计生物测定以确定暴露于IC的妊娠蚊子的死亡率和绝育效果。在不同容器中暴露的卵、幼虫和蛹中评估了抑制羽化的效果。通过反相高效液相色谱（RP-HPLC）测定活性成分的水安全评估浓度，并计算健康风险。应用于储水容器内壁的IC对埃及伊蚊显示出功效，表现为妊娠雌蚊高死亡率（24小时时为81%，p<0.01）、绝育效果（产卵抑制63%，p<0.01）和羽化抑制（卵、L3和L4中为100%；蛹中为97%）。后代率降低[每只暴露的妊娠雌蚊仅出现0.15只（38/250）新成虫，而基线时每只未暴露雌蚊出现11.90只（2976/250）]。羽化抑制记录长达12个月，成虫死亡率>80%长达6个月。使用储存在经过处理的容器中的水用于洗涤或饮用，预计不会对使用者构成健康风险。应用于家用储水容器的IC具有双重和互补作用，可降低埃及伊蚊密度（未成熟和成虫阶段）。这代表了哥伦比亚一项整群随机疗效试验的基线信息。
