Ghouri Muhammad Daniyal, Tariq Ayesha, Saleem Jabran, Muhaymin Abdul, Cai Rong, Chen Chunying
New Cornerstone Science Laboratory, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, Chinese Academy of Sciences (CAS), Beijing 100190, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Nanoscale Horiz. 2024 Dec 16;10(1):179-189. doi: 10.1039/d4nh00178h.
Nanoparticle interactions with biological systems are intricate processes influenced by various factors, among which the formation of protein corona plays a pivotal role. This research investigates a novel aspect of nanoprotein corona-cell interactions, focusing on the impact of the protein corona on the recovery of disrupted tight junctions in endothelial cells. We demonstrate that the protein corona formed on the surface of star-shaped nanoparticles induces the aggregates of ZO-1, which is quite important for the barriers' integrity. Our research emphasizes that the APOA1 pre-coating on the nanoparticles reduces the ZO-1 expression of endothelial cells offering a promising strategy for overcoming the bio barriers. These findings contribute to our understanding of the interplay between nanoparticles, protein corona, and endothelial cell junctions, offering insights for developing innovative therapeutic approaches targeting the blood-brain barrier integrity. Our study holds promise for the future of nanomedicine, nano drug delivery systems and development of strategies to mitigate potential adverse effects.
纳米颗粒与生物系统的相互作用是受多种因素影响的复杂过程，其中蛋白质冠层的形成起着关键作用。本研究调查了纳米蛋白质冠层与细胞相互作用的一个新方面，重点关注蛋白质冠层对内皮细胞中受损紧密连接恢复的影响。我们证明，星形纳米颗粒表面形成的蛋白质冠层会诱导ZO-1聚集，这对屏障的完整性非常重要。我们的研究强调，纳米颗粒上的APOA1预涂层可降低内皮细胞的ZO-1表达，为克服生物屏障提供了一种有前景的策略。这些发现有助于我们理解纳米颗粒、蛋白质冠层和内皮细胞连接之间的相互作用，为开发针对血脑屏障完整性的创新治疗方法提供了见解。我们的研究为纳米医学、纳米药物递送系统以及减轻潜在不良反应的策略开发带来了希望。
