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超高效液相色谱-四极杆飞行时间串联质谱法筛选和鉴定怀特,并对其抗氧化、抗癌和抗炎潜力进行体外评估。

UPLC-QToF-MS/MS screening and characterization of Wight and in vitro assessment of its antioxidant, anticancer, and anti-inflammatory potential.

作者信息

Sahoo Dibya Ranjan, Babu Swaraj Kumar, Naik Baishali Basundhara, Hota Sajna Sameekshya, Bhoi Namita, Sarkar Barun, Ali S K Mustaq, Naik Pradeep Kumar

机构信息

Centre of Excellence in Natural Products and Therapeutics, Department of Biotechnology and Bioinformatics, Sambalpur University, Jyoti Vihar, Burla, Sambalpur, Odisha 768 019 India.

Waters India Private Limited, Unit No. 902, 9th Floor, Merlin Infinite, DN 51, Sector-V, Salt Lake City, Kolkata, West Bengal 700091 India.

出版信息

3 Biotech. 2024 Dec;14(12):298. doi: 10.1007/s13205-024-04144-x. Epub 2024 Nov 12.

Abstract

belongs to the Lamiaceae family and is locally known as Badichang or mahasindhu. In this study, we performed Soxhlet extraction to prepare methanolic and hydromethanolic extracts, followed by quantification of their total phenolic content and total flavonoid content. Qualitative analysis of both the extracts was conducted to determine the presence of different phytochemicals. In addition, we aimed to identify the important phytochemical constituents in the methanolic extracts of (SPM) using ultra-performance liquid chromatography hyphenated with high-resolution mass spectrometry (UPLC-ESI-QTOF-MS). Furthermore, this study investigated the antioxidant, anticancer and anti-inflammatory properties of SPM and its safety profile in the normal fibroblast cell line L929. A colony proliferation assay and a Griess assay were performed to evaluate the effects of SPM on colony formation and nitric oxide (NO) production. A total of 13 important phytochemicals were identified and reported. The methanolic extract of SPM demonstrated significant antioxidant activity. SPM also showed substantial antiproliferative activity on MDA-MB-231 triple-negative breast cancer cells, with an IC value of 45.53 ± 1.63 µg/ml, and also reduced the survival of these cancer cells by promoting nuclear fragmentation and condensation without causing harm to normal cells. SPM inhibits the colony formation and reduces the nitric oxide (NO) production. The anti-inflammatory potential of SPM was assessed utilizing the murine alveolar macrophages (J774.A.1) as an in vitro model, and SPM effectively lowered the levels of proinflammatory cytokines such as TNF-α and IL-6. These findings emphasized the antiproliferative potential of SPM to cancer cells, along with its anti-inflammatory, and antioxidant capabilities, indicating the therapeutic efficacy of this medicinal plant.

摘要

属于唇形科,当地称为Badichang或mahasindhu。在本研究中,我们进行了索氏提取以制备甲醇提取物和氢甲醇提取物,随后对其总酚含量和总黄酮含量进行了定量。对两种提取物进行了定性分析,以确定不同植物化学物质的存在。此外,我们旨在使用超高效液相色谱与高分辨率质谱联用(UPLC-ESI-QTOF-MS)鉴定(SPM)甲醇提取物中的重要植物化学成分。此外,本研究还研究了SPM在正常成纤维细胞系L929中的抗氧化、抗癌和抗炎特性及其安全性。进行了集落增殖试验和Griess试验以评估SPM对集落形成和一氧化氮(NO)产生的影响。共鉴定并报道了13种重要的植物化学成分。SPM的甲醇提取物表现出显著的抗氧化活性。SPM对MDA-MB-231三阴性乳腺癌细胞也表现出显著的抗增殖活性,IC值为45.53±1.63μg/ml,并且通过促进核碎裂和凝聚降低了这些癌细胞的存活率,而不会对正常细胞造成伤害。SPM抑制集落形成并减少一氧化氮(NO)的产生。利用小鼠肺泡巨噬细胞(J774.A.1)作为体外模型评估了SPM的抗炎潜力,SPM有效地降低了促炎细胞因子如TNF-α和IL-6的水平。这些发现强调了SPM对癌细胞的抗增殖潜力及其抗炎和抗氧化能力,表明了这种药用植物的治疗效果。

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