Chen Gaoxiang, Wang Rongchang, Ying Luyao, Eheneden Iyobosa, Ren Haijing, Sun Maoxin
Institute of Biofilm Technology, Key Laboratory of Yangtze Aquatic Environment (MOE), College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, Shanghai, PR China.
Institute of Biofilm Technology, Key Laboratory of Yangtze Aquatic Environment (MOE), College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, Shanghai, PR China.
Environ Res. 2025 Jan 1;264(Pt 2):120365. doi: 10.1016/j.envres.2024.120365. Epub 2024 Nov 14.
Efficient removal of ammonia nitrogen and sulfamethoxazole (SMX) from wastewater has become increasingly critical due to their detrimental effects on aquatic ecosystems and public health. This study aimed to investigate the nitrogen transformation and SMX removal in a membrane aerated biofilm reactor (MABR) under different SMX concentrations (0-200 μg L) with a nitrifying membrane bioreactor (MBR) as a control. Results suggested that SMX removal in MABR was better than that of MBR with SMX addition (50-200 μg L). Membrane aerated biofilms tended to secrete more extracellular polymeric substances (EPS) and generate less antioxidant enzymes in response to SMX stress when compared with nitrifying sludge in MBR. Metagenomic analysis indicated that distinct succession of microbial community was observed in both systems after SMX addition, and the relative abundance of nitrifying bacteria (Nitrosomonas, Nitrospira, and Nitrobacter) evidently decreased under SMX concentration of 200 μg L. The proliferation of predominant antibiotic resistance gene (ARG) sul2 was suppressed more obviously in MABR than that in MBR. Thus, this study provided extensive insights into the advantages of nitrifying MABR in simultaneous removal of ammonium and antibiotics with less risk of associated ARGs spread.
由于氨氮和磺胺甲恶唑（SMX）对水生生态系统和公众健康具有有害影响，因此从废水中有效去除它们变得越来越重要。本研究旨在以硝化膜生物反应器（MBR）作为对照，研究在不同SMX浓度（0 - 200μg/L）下膜曝气生物膜反应器（MABR）中的氮转化和SMX去除情况。结果表明，添加SMX（50 - 200μg/L）时，MABR中SMX的去除效果优于MBR。与MBR中的硝化污泥相比，膜曝气生物膜在受到SMX胁迫时倾向于分泌更多的胞外聚合物（EPS）并产生更少的抗氧化酶。宏基因组分析表明，添加SMX后，两个系统中均观察到微生物群落的明显演替，在200μg/L的SMX浓度下，硝化细菌（亚硝化单胞菌、硝化螺菌和硝化杆菌）的相对丰度明显下降。在MABR中，主要抗生素抗性基因（ARG）sul2的增殖受到的抑制比在MBR中更明显。因此，本研究为硝化MABR在同时去除铵和抗生素方面的优势提供了广泛的见解，且相关抗生素抗性基因传播的风险较小。
