人补体成分C3天冬酰胺连接寡糖的结构分析
Structural analysis of the asparagine-linked oligosaccharides of human complement component C3.
作者信息
Hirani S, Lambris J D, Müller-Eberhard H J
出版信息
Biochem J. 1986 Jan 15;233(2):613-6. doi: 10.1042/bj2330613.
Abstract
The asparagine-linked oligosaccharides of human C3 were characterized. The C3 oligosaccharides were released by endo-beta-N-acetylglucosaminidase H and were analysed by lectin affinity chromatography and h.p.l.c. The released oligosaccharides bound tightly to concanavalin A-Sepharose and were not retained by agarose-bound wheat-germ agglutinin, indicating that they were only of high-mannose type. Two major oligosaccharide structures were separated from both the alpha- and beta-chains of C3 by h.p.l.c. on Micropak AX-5, calibrated with high-mannose-type oligosaccharides of known structures. The oligosaccharide structures on the alpha-chain have the compositions (Man)9(GlcNAc)2-Asn and (Man)8 (GlcNAc)2-Asn, and those on the beta-chain have the compositions (Man)6(GlcNAc)2-Asn and (Man)5(GlcNAc)2-Asn.
摘要
对人补体C3的天冬酰胺连接的寡糖进行了表征。补体C3寡糖经内切β-N-乙酰葡糖胺糖苷酶H释放后，通过凝集素亲和色谱法和高效液相色谱法进行分析。释放出的寡糖与伴刀豆球蛋白A-琼脂糖紧密结合，而不被琼脂糖结合的麦胚凝集素保留，这表明它们仅为高甘露糖型。通过在Micropak AX-5上进行高效液相色谱法，用已知结构的高甘露糖型寡糖进行校准，从补体C3的α链和β链中分离出两种主要的寡糖结构。α链上的寡糖结构组成为（甘露糖）9（N-乙酰葡糖胺）2-天冬酰胺和（甘露糖）8（N-乙酰葡糖胺）2-天冬酰胺，β链上的寡糖结构组成为（甘露糖）6（N-乙酰葡糖胺）2-天冬酰胺和（甘露糖）5（N-乙酰葡糖胺）2-天冬酰胺。
相似文献
1
Structural analysis of the asparagine-linked oligosaccharides of human complement component C3.人补体成分C3天冬酰胺连接寡糖的结构分析
Biochem J. 1986 Jan 15;233(2):613-6. doi: 10.1042/bj2330613.
2
Asn-linked oligosaccharides in lectin-resistant tumor-cell mutants with varying metastatic potential.具有不同转移潜能的凝集素抗性肿瘤细胞突变体中的天冬酰胺连接的寡糖。
Eur J Biochem. 1986 Dec 1;161(2):359-73. doi: 10.1111/j.1432-1033.1986.tb10455.x.
3
4
Developmental regulation of asparagine-linked oligosaccharide synthesis in Dictyostelium discoideum.盘基网柄菌中天冬酰胺连接型寡糖合成的发育调控
J Biol Chem. 1991 Oct 5;266(28):18485-97.
5
Carbohydrate structures of the third component of rat complement. Presence of both high-mannose and complex type oligosaccharide chains.大鼠补体第三成分的碳水化合物结构。同时存在高甘露糖型和复合型寡糖链。
Biochem J. 1986 Dec 15;240(3):691-8. doi: 10.1042/bj2400691.
6
Analysis of asparagine-linked oligosaccharide structures of chronic lymphocytic leukemia cells.慢性淋巴细胞白血病细胞中天冬酰胺连接寡糖结构的分析
Mol Immunol. 1987 Aug;24(8):871-86. doi: 10.1016/0161-5890(87)90190-8.
7
Structures of galactose-containing oligosaccharides of alpha-mannosidase from porcine kidney.猪肾α-甘露糖苷酶含半乳糖寡糖的结构
J Biochem. 1986 Aug;100(2):505-12. doi: 10.1093/oxfordjournals.jbchem.a121740.
8
9
Asparagine-linked oligosaccharides of BHK cells treated with inhibitors of oligosaccharide processing.用寡糖加工抑制剂处理的BHK细胞的天冬酰胺连接寡糖
Biochem J. 1987 Nov 1;247(3):537-45. doi: 10.1042/bj2470537.
10
Characterization of the high mannose asparagine-linked oligosaccharides synthesized by Schistosoma mansoni adult male worms.曼氏血吸虫成年雄虫合成的高甘露糖型天冬酰胺连接寡糖的特性分析
Mol Biochem Parasitol. 1988 Apr;28(3):265-74. doi: 10.1016/0166-6851(88)90011-4.
引用本文的文献
1
Glycosylation as an intricate post-translational modification process takes part in glycoproteins related immunity.糖基化作为一个复杂的翻译后修饰过程，参与了与糖蛋白相关的免疫反应。
Cell Commun Signal. 2025 May 5;23(1):214. doi: 10.1186/s12964-025-02216-w.
2
Charting the Proteoform Landscape of Serum Proteins in Individual Donors by High-Resolution Native Mass Spectrometry.采用高分辨天然质谱法描绘个体供体血清蛋白质的蛋白形式图谱。
Anal Chem. 2022 Sep 20;94(37):12732-12741. doi: 10.1021/acs.analchem.2c02215. Epub 2022 Sep 8.
3
High-Throughput Human Complement C3 N-Glycoprofiling Identifies Markers of Early Onset Type 1 Diabetes Mellitus in Children.高通量人类补体 C3 N-糖基化分析鉴定儿童早发 1 型糖尿病的标志物。
Mol Cell Proteomics. 2022 Oct;21(10):100407. doi: 10.1016/j.mcpro.2022.100407. Epub 2022 Aug 27.
4
The role of protein glycosylation in muscle diseases.蛋白质糖基化在肌肉疾病中的作用。
Mol Biol Rep. 2022 Aug;49(8):8037-8049. doi: 10.1007/s11033-022-07334-z. Epub 2022 Apr 15.
5
The crystal structure of iC3b-CR3 αI reveals a modular recognition of the main opsonin iC3b by the CR3 integrin receptor.iC3b-CR3αI 的晶体结构揭示了主要调理素 iC3b 被 CR3 整合素受体模块化识别。
Nat Commun. 2022 Apr 12;13(1):1955. doi: 10.1038/s41467-022-29580-2.
6
Uptakes Carbohydrate Source from Host Glycoproteins by N-glycans Degradation System for Optimal Survival and Full Virulence during Infection.通过 N-聚糖降解系统从宿主糖蛋白摄取碳水化合物来源，以在感染期间实现最佳存活和完全毒力。
Pathogens. 2020 May 18;9(5):387. doi: 10.3390/pathogens9050387.
7
Atomic-resolution crystal structures of the immune protein conglutinin from cow reveal specific interactions of its binding site with -acetylglucosamine.牛免疫蛋白血纤维蛋白原的原子分辨率晶体结构揭示了其结合位点与 N-乙酰葡萄糖胺的特定相互作用。
J Biol Chem. 2019 Nov 8;294(45):17155-17165. doi: 10.1074/jbc.RA119.010271. Epub 2019 Sep 27.
8
Structural Implications for the Formation and Function of the Complement Effector Protein iC3b.补体效应蛋白iC3b形成与功能的结构影响
J Immunol. 2017 Apr 15;198(8):3326-3335. doi: 10.4049/jimmunol.1601864. Epub 2017 Mar 3.
9
Molecular Characterization of N-glycan Degradation and Transport in Streptococcus pneumoniae and Its Contribution to Virulence.肺炎链球菌中N-聚糖降解与转运的分子特征及其对毒力的贡献
PLoS Pathog. 2017 Jan 5;13(1):e1006090. doi: 10.1371/journal.ppat.1006090. eCollection 2017 Jan.
10
Complement component C3 - The "Swiss Army Knife" of innate immunity and host defense.补体成分C3——固有免疫和宿主防御的“瑞士军刀”。
Immunol Rev. 2016 Nov;274(1):33-58. doi: 10.1111/imr.12500.
本文引用的文献
1
CONGLUTINATION: SPECIFIC INHIBITION BY CARBOHYDRATES.共凝集作用：碳水化合物的特异性抑制作用
Science. 1964 Mar 20;143(3612):1327-8.
2
Separation of neutral oligosaccharides by high-performance liquid chromatography.通过高效液相色谱法分离中性寡糖。
Anal Biochem. 1981 Jul 1;114(2):276-80. doi: 10.1016/0003-2697(81)90480-2.
3
The carbohydrate-binding specificity of pea and lentil lectins. Fucose is an important determinant.豌豆和小扁豆凝集素的碳水化合物结合特异性。岩藻糖是一个重要的决定因素。
J Biol Chem. 1981 Jul 10;256(13):6633-40.
4
Biosynthesis and processing of a human precursor complement protein, pro-C3, in a hepatoma-derived cell line.一种人类补体前体蛋白——C3前体在肝癌衍生细胞系中的生物合成与加工
Science. 1982 Jan 22;215(4531):399-400. doi: 10.1126/science.7199205.
5
Preparation and fractionation of glycopeptides.糖肽的制备与分级分离
Methods Enzymol. 1982;83:269-77. doi: 10.1016/0076-6879(82)83020-6.
6
Structural requirements for the binding of oligosaccharides and glycopeptides to immobilized wheat germ agglutinin.寡糖和糖肽与固定化麦胚凝集素结合的结构要求。
Biochemistry. 1981 Sep 29;20(20):5894-9. doi: 10.1021/bi00523a037.
7
8
Release of endogenous C3b inactivator from lymphocytes in response to triggering membrane receptors for beta 1H globulin.响应β1H球蛋白的触发膜受体，淋巴细胞释放内源性C3b灭活剂。
J Exp Med. 1980 Dec 1;152(6):1625-44. doi: 10.1084/jem.152.6.1625.
9
Catabolites of the third component of complement in urines of hereditary nephritis patients.遗传性肾炎患者尿液中补体第三成分的分解代谢产物
J Biol Chem. 1983 Jun 10;258(11):7200-7.
Zotero 插件