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网络药理学和体外实验通过RAS/MAPK信号通路研究芍药苷的抗胃癌作用。

Network pharmacology and in vitro experiments to investigate the anti-gastric cancer effects of paeoniflorin through the RAS/MAPK signaling pathway.

作者信息

Yang Yating, Yuan Ling, Du Yuhua, Ye Mengyi, Lu Doudou, Huang Shicong, Zhao Jianjun, Tibenda Joanna Japhet, Meng Fandi, Nan Yi

机构信息

Traditional Chinese Medicine College, Ningxia Medical University, Yinchuan, 750004, Ningxia Hui Autonomous, China.

College of Pharmacy, Ningxia Medical University, Yinchuan, 750004, Ningxia Hui Autonomous Region, China.

出版信息

Discov Oncol. 2024 Nov 15;15(1):659. doi: 10.1007/s12672-024-01532-w.

DOI:10.1007/s12672-024-01532-w
PMID:39548020
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11568095/
Abstract

The aim of this study was to investigate the key targets and signaling pathways of paeoniflorin (PF) for the treatment of gastric cancer (GC). First, the differentially expressed genes (DEGs) of gastric cancer were obtained by analyzing GSE118916 Gene Chip, and then the active components of paeoniflorin and their targets of action were found. And the intersection genes of the two were analyzed for target and pathway analysis. In addition, cell viability after PF intervention was detected by CCK-8. Clone formation assay, wound scratch assay, transwell assay were used to detect cell migration and invasion. The qRT-PCR and Western blot methods were used to verify the mechanism of action. The results showed that a total of 286 paeoniflorin targets and 1799 DEGs were obtained. Secondly, we found that PF could treat gastric cancer through RAS/MAPK signaling pathway. In addition, through in vitro cellular experiments, we also found that PF had a significant therapeutic effect on gastric cancer. Therefore, we believe that PF inhibits the proliferation and metastasis of gastric cancer, and its effect may be exerted by regulating the RAS/MAPK signaling pathway. PF is a promising drug for the treatment of gastric cancer. Combined with the in vitro experiments, we found that the therapeutic effect of PF is related to the regulation of the RAS/MAPK signaling pathway, and the results of the present study preliminarily revealed its complex mechanism, which will lay the foundation for future clinical treatment.

摘要

本研究旨在探讨芍药苷（PF）治疗胃癌（GC）的关键靶点及信号通路。首先，通过分析GSE118916基因芯片获得胃癌的差异表达基因（DEGs），然后找出芍药苷的活性成分及其作用靶点。并对两者的交集基因进行靶点和通路分析。此外，采用CCK-8检测PF干预后的细胞活力。采用克隆形成实验、划痕实验、transwell实验检测细胞迁移和侵袭能力。运用qRT-PCR和蛋白质免疫印迹法验证其作用机制。结果显示，共获得286个芍药苷靶点和1799个DEGs。其次，发现PF可通过RAS/MAPK信号通路治疗胃癌。此外，通过体外细胞实验，还发现PF对胃癌有显著治疗作用。因此，认为PF可抑制胃癌的增殖和转移，其作用可能是通过调节RAS/MAPK信号通路来实现的。PF是一种有前景的胃癌治疗药物。结合体外实验，发现PF的治疗作用与RAS/MAPK信号通路的调节有关，本研究结果初步揭示了其复杂机制，为今后的临床治疗奠定了基础。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80bc/11568095/902dbf5f5890/12672_2024_1532_Fig10_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80bc/11568095/d3495272faed/12672_2024_1532_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80bc/11568095/e3a59d550453/12672_2024_1532_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80bc/11568095/614af4c75330/12672_2024_1532_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80bc/11568095/10d1f417a923/12672_2024_1532_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80bc/11568095/c25caee5f5ba/12672_2024_1532_Fig9_HTML.jpg
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