Sam Gayathri, Chen Shuxiong, Rehm Bernd H A
Centre for Cell Factories and Biopolymers (CCFB), Institute for Biomedicine and Glycomics, Griffith University (Nathan Campus), QLD 4111, Australia.
Centre for Cell Factories and Biopolymers (CCFB), Institute for Biomedicine and Glycomics, Griffith University (Nathan Campus), QLD 4111, Australia.
N Biotechnol. 2025 Mar 25;85:9-15. doi: 10.1016/j.nbt.2024.11.002. Epub 2024 Nov 15.
Polyhydroxybutyrate (PHB) is a biodegradable and biocompatible biopolyester, naturally produced and self-assembled as spherical inclusions inside bacteria. These PHB particles contain a hydrophobic PHB core covalently coated with PHB synthase (PhaC), which serves as an anchoring linker for foreign proteins of interest. Protein engineering of PhaC enables the display of biologically active protein functions on the surface of PHB particles suitable for different applications. Many biomolecules, such as e.g. antigens, enzymes, fluorescent proteins were immobilized to PHB particles and exhibited superior functionalities when compared to their respective soluble counterparts. Recently, PHB particles have been successfully applied for various diagnostics applications. This mini review provides an overview of the unique design space of PHB particles towards the development of safe and cost-effective diagnostic tools, and highlights the important research progresses of manufacturing PHB particles-based diagnostics.
聚羟基丁酸酯（PHB）是一种可生物降解且具有生物相容性的生物聚酯，它在细菌内部自然产生并自组装成球形内含物。这些PHB颗粒包含一个疏水的PHB核心，该核心共价包被有PHB合酶（PhaC），PhaC作为感兴趣的外源蛋白的锚定连接物。对PhaC进行蛋白质工程改造能够在适合不同应用的PHB颗粒表面展示生物活性蛋白功能。许多生物分子，例如抗原、酶、荧光蛋白，都被固定在PHB颗粒上，并且与各自的可溶性对应物相比表现出更优异的功能。最近，PHB颗粒已成功应用于各种诊断应用。这篇小型综述概述了PHB颗粒在开发安全且经济高效的诊断工具方面的独特设计空间，并突出了基于PHB颗粒的诊断方法制造方面的重要研究进展。
