Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, US 41-UAR 2014-PLBS, 59000, Lille, France.
Inserm U1170, 94800, Villejuif, France.
Sci Rep. 2024 Nov 16;14(1):28297. doi: 10.1038/s41598-024-79307-0.
The RhoH protein is a member of the Ras superfamily of guanosine triphosphate-binding proteins. RhoH is an atypical Rho family member that is always GTP-bound and thus always activated. It is restrictively expressed in normal hematopoietic cells, where it is a negative regulator of cell growth and survival. We previously analyzed the RHOH gene structure and demonstrated that this gene is composed of 7 exons, one single encoding exon located at the 3' extremity of the gene, preceded by 6 noncoding exons. To further understand the transcription events associated with this gene, we performed full-length RNA-Seq on 12 B-cell lines. We identified new exons, new splice events and new splice sites, leading to the discovery of 38 RHOH mRNA molecules, 27 of which have never been described before. Here, we also describe new fusion transcripts. Moreover, our method allowed quantitative measurements of the different mRNA species relative to each other in relation to B-cell differentiation.
RhoH 蛋白是 Ras 家族鸟嘌呤三磷酸结合蛋白的成员。RhoH 是一种非典型的 Rho 家族成员，它总是与 GTP 结合，因此总是处于激活状态。它在正常造血细胞中受到限制表达，在造血细胞中，它是细胞生长和存活的负调节剂。我们之前分析了 RHOH 基因结构，并证明该基因由 7 个外显子组成，一个单一的编码外显子位于基因的 3'末端，前面有 6 个非编码外显子。为了进一步了解与该基因相关的转录事件，我们对 12 个 B 细胞系进行了全长 RNA-Seq 分析。我们鉴定了新的外显子、新的剪接事件和新的剪接位点，从而发现了 38 种 RHOH mRNA 分子，其中 27 种以前从未描述过。在这里，我们还描述了新的融合转录本。此外，我们的方法允许定量测量与 B 细胞分化有关的不同 mRNA 种彼此之间的相对关系。
