The global prevalence of microsporidia infection in rabbits as a neglected public health concern: A systematic review and meta-analysis.

Microsporidia are intracellular parasites with significant impact on both animal and human health. The prevalence of microsporidia infections in rabbits, including the genera Enterocytozoon and Encephalitozoon, underscores the importance of understanding their epidemiology for effective control strategies. This systematic review and meta-analysis estimated the global prevalence of microsporidia infection in rabbits using five databases (Scopus, PubMed, Embase, Web of Science, and Google Scholar) to retrieve articles published between 03 December 2003 and 26 March 2023. The global prevalence was estimated with a 95 % confidence interval. All statistical analyses conducted were based on meta-package of R (version 3.6.1). A p-value lower than 0.05 was interpreted as statistically significant. A total of 71 studies comprising 72 datasets were included, yielding a global pooled prevalence of microsporidia infections in rabbits at 0.312 (0.250-0.378). The prevalence varied significantly by continent with highest observed in North America (0.495, 0.151-0.842). Slovenia had the highest pooled prevalence (0.714, 0.654-0.773). Encephalitozoon cuniculi accounted for the highest prevalence (0.338, 0.271-0.407). The findings highlight the global distribution of microsporidia in rabbit populations, emphasizing the zoonotic potential and public health implications. The predominance of E. cuniculi underscores its importance as a widespread pathogen affecting both animal and human health. The data underscore the need for continued surveillance and monitoring, particularly in regions with high prevalence.