Peng Jianlan, Liu Chang, Mo Meilan, Huang Yu, Lu Yeqing, Xiao Mengjie, Zhao Xin, Ruan Qijun, Ti Huihui
School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Institute of Analysis, Guangdong Academy of Sciences (China National Analytical Center, Guangzhou), Guangzhou 510070, China.
Int J Biol Macromol. 2024 Dec;283(Pt 2):137746. doi: 10.1016/j.ijbiomac.2024.137746. Epub 2024 Nov 16.
Nanozymes have become promising alternative antibacterial agents for bacteria-infected wounds. In this study, fucoidan-confined gold nanoparticles (Fuc@AuNPs) are developed by in situ reduction, and stabilized by sulfate groups of fucoidan. Fuc@AuNPs exhibit pH-responsive catalytic activity that can mimic oxidase (OXD) under acidic bacterial infection conditions and mimic superoxide dismutase (SOD) under normal physiological conditions. The OXD-like catalytic activity of Fuc@AuNPs generates active singlet oxygen (O), exhibiting effective antibacterial properties against both Gram-negative E. coli and Gram-positive S. aureus. Fuc@AuNPs and aldehyde grafted saponin incorporate with chitosan to form a hybrid hydrogel. This hydrogel exhibits superior mechanical, adhesive, and self-healing properties due to electrostatic complex coacervation networks and dynamic covalent Schiff base reactions. Animal experiments show that the hydrogel aids S. aureus-infected skin wound healing by reducing bacterial infection and promoting granulation tissue formation without causing excessive ROS-induced inflammation. This study presents the design of multifunctional nanozymes and bioactive hydrogels as a promising wound healing dressing for biomedical applications.
纳米酶已成为治疗细菌感染伤口的一种很有前景的新型抗菌剂。在本研究中，通过原位还原法制备了岩藻依聚糖包裹的金纳米颗粒（Fuc@AuNPs），并通过岩藻依聚糖的硫酸基团使其稳定。Fuc@AuNPs表现出pH响应催化活性，在酸性细菌感染条件下可模拟氧化酶（OXD），在正常生理条件下可模拟超氧化物歧化酶（SOD）。Fuc@AuNPs的类氧化酶催化活性产生活性单线态氧（O），对革兰氏阴性大肠杆菌和革兰氏阳性金黄色葡萄球菌均表现出有效的抗菌性能。Fuc@AuNPs和醛基接枝皂苷与壳聚糖结合形成一种混合水凝胶。由于静电复合凝聚网络和动态共价席夫碱反应，这种水凝胶具有优异的机械性能、粘附性能和自愈性能。动物实验表明，该水凝胶通过减少细菌感染和促进肉芽组织形成，有助于金黄色葡萄球菌感染的皮肤伤口愈合，且不会引起过多的活性氧诱导的炎症。本研究提出了多功能纳米酶和生物活性水凝胶的设计，作为一种有前途的生物医学应用伤口愈合敷料。
