Hood Robert B, Liang Donghai, Wang Yilin, Tan Youran, Souter Irene, Jones Dean P, Hauser Russ, Chavarro Jorge E, Gaskins Audrey J
Department of Epidemiology, Emory University, Rollins School of Public Health, Atlanta, GA, United States.
Department of Epidemiology, Emory University, Rollins School of Public Health, Atlanta, GA, United States; Gangarosa Department of Environmental Health, Emory University, Rollins School of Public Health, Atlanta, GA, United States.
J Nutr. 2025 Feb;155(2):559-569. doi: 10.1016/j.tjnut.2024.11.013. Epub 2024 Nov 16.
The profertility diet is a dietary pattern composed of nutrients and foods most consistently associated with in vitro fertilization (IVF) end points in women.
We examined the potential biological mechanisms underlying the association between adherence to a profertility diet and IVF end points using high-resolution metabolomics.
Among 120 women who underwent an autologous oocyte IVF cycle (2007-2015) in Northeast United States, we collected a serum sample during controlled ovarian stimulation and a follicular fluid sample on the day of oocyte retrieval. Women completed a food frequency questionnaire upon enrollment into the study to examine adherence to the profertility diet pattern. Liquid chromatography with high-resolution mass spectrometry was used for untargeted metabolomic analysis of biospecimens. We identified metabolic features (and enriched biological pathways) associated with the profertility diet and 2 IVF end points, live birth and clinical pregnancy, via a meet-in-the-middle approach.
In the follicular fluid metabolome, vitamin D-3 metabolism was associated with adherence to the profertility diet pattern and live birth. Additionally, vitamin D-3 metabolism, vitamin B-6 metabolism, and bile acid biosynthesis were associated with both adherence to the profertility diet pattern and clinical pregnancy. In the serum metabolome, only tryptophan metabolism was associated with adherence to the profertility diet pattern and live birth. We confirmed the chemical identity of a metabolite with level 1 evidence, 4-pyridoxate, which was higher in the serum and follicular fluid among women with stronger adherence to the profertility diet pattern and among women with a live birth.
The beneficial association between adherence to the profertility diet and IVF outcomes may be mediated through vitamin D-3 metabolism, vitamin B-6 metabolism, and bile acid biosynthesis in the follicular fluid and tryptophan metabolism in the serum. These results provide new insight in the important biological pathways underlying a dietary pattern providing optimal fertility benefits to women.
生育促进饮食是一种由与女性体外受精（IVF）终点最密切相关的营养素和食物组成的饮食模式。
我们使用高分辨率代谢组学研究了坚持生育促进饮食与IVF终点之间关联的潜在生物学机制。
在美国东北部120名接受自体卵母细胞IVF周期治疗（2007 - 2015年）的女性中，我们在控制性卵巢刺激期间采集了血清样本，并在取卵当天采集了卵泡液样本。女性在进入研究时完成了一份食物频率问卷，以检查对生育促进饮食模式的依从性。采用液相色谱与高分辨率质谱联用技术对生物样本进行非靶向代谢组学分析。我们通过中间相遇法确定了与生育促进饮食以及两个IVF终点（活产和临床妊娠）相关的代谢特征（和富集的生物学途径）。
在卵泡液代谢组中，维生素D - 3代谢与对生育促进饮食模式的依从性及活产相关。此外，维生素D - 3代谢、维生素B - 6代谢和胆汁酸生物合成与对生育促进饮食模式的依从性及临床妊娠均相关。在血清代谢组中，只有色氨酸代谢与对生育促进饮食模式的依从性及活产相关。我们以1级证据确认了一种代谢物4 - 吡哆酸的化学身份，在对生育促进饮食模式依从性更强的女性以及有活产的女性的血清和卵泡液中，该代谢物水平更高。
坚持生育促进饮食与IVF结局之间的有益关联可能通过卵泡液中的维生素D - 3代谢、维生素B - 6代谢和胆汁酸生物合成以及血清中的色氨酸代谢介导。这些结果为一种为女性提供最佳生育益处的饮食模式背后的重要生物学途径提供了新的见解。
